Anti-ß-phosphoglucomutase antibody (Biotin) (ab34541)
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Overview
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Product nameAnti-ß-phosphoglucomutase antibody (Biotin)
See all ß-phosphoglucomutase primary antibodies -
DescriptionGoat polyclonal to ß-phosphoglucomutase (Biotin)
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Host speciesGoat
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ConjugationBiotin
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Tested applicationsSuitable for: Immunomicroscopy, WB, ELISA, Dot blotmore details
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Species reactivityReacts with Lactococcus lacti
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Immunogen
Full length protein [Lactococcus lacti]
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General notes
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2 -
Concentration information loading... -
PurityIgG fraction
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Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab34541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunomicroscopy | ||
| WB | ||
| ELISA | ||
| Dot blot |
Application notes
Dot: Use at an assay dependent dilution.
ELISA: 1/4000 - 1/20000. This product has been assayed against 1.0 ug of beta Phosphoglucomutase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 62 kDa.
Suitable for antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/4000 - 1/20000. This product has been assayed against 1.0 ug of beta Phosphoglucomutase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 62 kDa.
Suitable for antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevanceß-phosphoglucomutase is an enzyme that transfers a phosphoryl group on a glucose monomer from the 1' to the 6' position in the forward direction or the 6' to the 1' position in the reverse. Specifically, it converts ß-D-glucose-1-phosphate to ß-D-glucose-6-phosphate. This enzyme participates in both the breakdown and synthesis of glucose. Maltose metabolism in Lactococcus lactis involves the conversion of beta-glucose 1-phosphate to glucose 6-phosphate, a reaction which is reversibly catalysed by a maltose-inducible and glucose-repressible beta-phosphoglucomutase (beta-PGM). Alpha-PGM is expressed constitutively. ß-phosphoglucomutase is a member of the haloacid dehalogenase superfamily of hydrolase enzymes. The enzyme from Lactococcus lactis has been extensively characterised including a remarkable crystal structure which traps the pentacoordinate transition state.
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Cellular localizationCytoplasmic
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Alternative names
- Beta PGM antibody
- Beta-phosphoglucomutase antibody
- Glucose phosphomutase 1 antibody
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Protocols
References
ab34541 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"