Anti-ß-phosphoglucomutase antibody (Biotin) (ab34541)

Overview

  • Product name
    Anti-ß-phosphoglucomutase antibody (Biotin)
    See all ß-phosphoglucomutase primary antibodies
  • Description
    Goat polyclonal to ß-phosphoglucomutase (Biotin)
  • Host species
    Goat
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Immunomicroscopy, WB, ELISA, Dot blotmore details
  • Species reactivity
    Reacts with Lactococcus lacti
  • Immunogen

    Full length protein [Lactococcus lacti]

  • General notes

    Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule

Properties

Applications

Our Abpromise guarantee covers the use of ab34541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
WB
ELISA
Dot blot
  • Application notes
    Dot: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/20000. This product has been assayed against 1.0 ug of beta Phosphoglucomutase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 62 kDa.
    Suitable for antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      ß-phosphoglucomutase is an enzyme that transfers a phosphoryl group on a glucose monomer from the 1' to the 6' position in the forward direction or the 6' to the 1' position in the reverse. Specifically, it converts ß-D-glucose-1-phosphate to ß-D-glucose-6-phosphate. This enzyme participates in both the breakdown and synthesis of glucose. Maltose metabolism in Lactococcus lactis involves the conversion of beta-glucose 1-phosphate to glucose 6-phosphate, a reaction which is reversibly catalysed by a maltose-inducible and glucose-repressible beta-phosphoglucomutase (beta-PGM). Alpha-PGM is expressed constitutively. ß-phosphoglucomutase is a member of the haloacid dehalogenase superfamily of hydrolase enzymes. The enzyme from Lactococcus lactis has been extensively characterised including a remarkable crystal structure which traps the pentacoordinate transition state.
    • Cellular localization
      Cytoplasmic
    • Alternative names
      • Beta PGM antibody
      • Beta-phosphoglucomutase antibody
      • Glucose phosphomutase 1 antibody
      • Glucose phosphomutase antibody
      • PGM antibody
      see all

    References

    ab34541 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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