Anti-ß-phosphoglucomutase antibody (Biotin) (ab34541)

Overview

• Product name
  Anti-ß-phosphoglucomutase antibody (Biotin)
  See all ß-phosphoglucomutase primary antibodies
• Description
  Goat polyclonal to ß-phosphoglucomutase (Biotin)
• Conjugation
  Biotin
• Tested applications
  Suitable for: Immunomicroscopy, WB, ELISA, Dot blotmore details
• Species reactivity
  Reacts with Lactococcus lacti
• Immunogen

  Full length protein [Lactococcus lacti]

• General notes

  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  Preservative: 0.01% Sodium Azide
  Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
• Concentration information loading...
• Purity
  IgG fraction
• Purification notes
  This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Metabolism
  • Types of disease
  • Cancer

Associated products

• Compatible Secondaries
  • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
  • Donkey Anti-Goat IgG H&L (HRP) (ab205723)

Applications

Target

• Relevance
  ß-phosphoglucomutase is an enzyme that transfers a phosphoryl group on a glucose monomer from the 1' to the 6' position in the forward direction or the 6' to the 1' position in the reverse. Specifically, it converts ß-D-glucose-1-phosphate to ß-D-glucose-6-phosphate. This enzyme participates in both the breakdown and synthesis of glucose. Maltose metabolism in Lactococcus lactis involves the conversion of beta-glucose 1-phosphate to glucose 6-phosphate, a reaction which is reversibly catalysed by a maltose-inducible and glucose-repressible beta-phosphoglucomutase (beta-PGM). Alpha-PGM is expressed constitutively. ß-phosphoglucomutase is a member of the haloacid dehalogenase superfamily of hydrolase enzymes. The enzyme from Lactococcus lactis has been extensively characterised including a remarkable crystal structure which traps the pentacoordinate transition state.
• Cellular localization
  Cytoplasmic
• Alternative names
  • Beta PGM antibody
  • Beta-phosphoglucomutase antibody
  • Glucose phosphomutase 1 antibody

  • Glucose phosphomutase antibody
  • PGM antibody

  see all

Protocols

• Western blot protocols