Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesDot: Use at an assay dependent dilution.
ELISA: 1/4000 - 1/20000. This product has been assayed against 1.0 ug of beta Phosphoglucomutase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 62 kDa.
Suitable for antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Relevanceß-phosphoglucomutase is an enzyme that transfers a phosphoryl group on a glucose monomer from the 1' to the 6' position in the forward direction or the 6' to the 1' position in the reverse. Specifically, it converts ß-D-glucose-1-phosphate to ß-D-glucose-6-phosphate.
This enzyme participates in both the breakdown and synthesis of glucose.
Maltose metabolism in Lactococcus lactis involves the conversion of beta-glucose 1-phosphate to glucose 6-phosphate, a reaction which is reversibly catalysed by a maltose-inducible and glucose-repressible beta-phosphoglucomutase (beta-PGM). Alpha-PGM is expressed constitutively.
ß-phosphoglucomutase is a member of the haloacid dehalogenase superfamily of hydrolase enzymes. The enzyme from Lactococcus lactis has been extensively characterised including a remarkable crystal structure which traps the pentacoordinate transition state.
Beta PGM antibody
Glucose phosphomutase 1 antibody
Glucose phosphomutase antibody
References for Anti-ß-phosphoglucomutase antibody (Biotin) (ab34541)
has not yet been referenced specifically in any publications.
Publishing research using ab34541? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"