Complement C3a des Arg Human ELISA Kit (ab133037)

Overview

• Product nameComplement C3a des Arg Human ELISA Kit
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  High conc.	16	12.3ng/ml		11.1%
  Low conc.	16	1.86ng/ml		8.7%
  Medium conc.	16	3.4ng/ml		9.8%
• Sample type
  Plasma
• Assay typeCompetitive
• Sensitivity
  0.12 ng/ml
• Range
  0.313 ng/ml - 20 ng/ml
• Recovery

  Sample specific recovery

  Sample type	Average %	Range
  Plasma	94.8	% - %

• Assay time
  3h 00m
• Species reactivity
  Reacts with: Human
  
• Product overview

  ab133037 is a competitive immunoassay for the quantitative determination of C3a des Arg in plasma. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to Human C3a des Arg to bind, in a competitive manner, the Human C3a des Arg in the sample or an alkaline phosphatase molecule which has Human C3a des Arg attached to it. After incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation, the enzyme reaction is stopped and the yellow color generated is read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of Human C3a des Arg in either standards or samples. The measured optical density is used to calculate the concentration of Human C3a des Arg.  

• Notes

  The Human C3a des Arg molecule is one of three activation fragments formed from the activation of the complement cascade. C3a des Arg is formed from C3a via carboxypeptidase cleavage of the C-terminal arginine group. The structure of Human C3a des Arg was first reported in 1975 by Hugli. Human C3a des Arg contains 77 amino acids with 6 cysteines involved in disulfide bridges between residues 22-49, 23-56 and 36-57. The C terminal end of C3a des Arg in Human, porcine, rat, mouse and guinea pig is identical. C3a des Arg is a highly cationic molecule containing no carbohydrate. X-ray crystal data shows the N and C terminal residues to have highly flexible helical structures. C3a is one of the most potent constrictors of smooth muscle cells, and guinea pig airways are hyperresponsive to C3a when pretreated with histamine. The long term study of liver and other transplant recipients for both C3a des Arg and C4a des Arg may be useful in assessing a number of pathological conditions. The use of potent protease inhibitors, such as Futhan, in conjunction with EDTA, may allow complement activation factors to be quantitated specifically via inhibition of non-specific protease formation of C3a des Arg.

  Cross Reactivity

  Compound	Cross Reactivity
  Human Complement C3a des Arg	100%
  Human Complement C3	1.28%
  Human Complement C4a des Arg	0.40%
  Human Complement C4	0.31%
  Human Complement C5 des Arg	0.10%
  Human Complement C5	0.05%

   

• Tested applicationsCompetitive ELISA more details

Properties

• Storage instructionsPlease refer to protocols.

• Components	1 x 96 tests
  Assay Buffer 10 Concentrate	1 x 15ml
  Complement Reagent A	1 x 15ml
  Complement Reagent B	1 x 30ml
  Goat anti-Rabbit IgG Microtiter Plate	1 unit
  human C3a des Arg Antibody	1 x 6ml
  human C3a des Arg Conjugate	1 x 6ml
  human C3a des Arg Standard	2 x 500ng
  Plate Sealer	2 units
  pNpp Substrate	1 x 23ml
  Stop Solution	1 x 6ml
  Wash Buffer Concentrate	1 x 30ml

• Research Areas
  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Complement ELISA kits

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Blood coagulation ELISA kits

  • Immunology
  • Innate Immunity
  • Complement
  • Alternative Pathway

  • Immunology
  • Innate Immunity
  • Complement
  • Classical Pathway

Applications

Complement C3a des Arg Human ELISA Kit images

• 

  Human C3a des Arg Standard Curve
  This curve must not be used to calculate Human C3a des Arg concentrations; each user must run a standard curve for each assay.

Protocols

Protocol Booklet