Overview
- Product nameIgG2a Mouse ELISA Kit
- Precision
Intra-assay Sample n Mean SD CV% Low IgG2a 16 43.4ng/ml 1.8% Medium IgG2a 16 69.8ng/ml 1.2% High IgG2a 16 112ng/ml 2.1% - Sample typeSerum, Tissue Culture Media
- Assay typeSandwich
- Sensitivity318.8 pg/ml
- Range7.81 ng/ml - 250 ng/ml
- Recovery
Sample specific recovery Sample type Average % Range Serum = 98.4 % - % Tissue Culture Media = 98.2 % - % - Species reactivityReacts with: Mouse
- Product overview
ab133046 is a complete kit for the quantitative determintation of mouse IgG2a in Tissue Culture Media and serum. Please read the complete kit insert before performing this assay.The kit uses a polyclonal antibody to mouse IgG immobilized on a microtiter plate to bind the mouse IgG in the standards or sample. A mouse IgG2a Standard is provided in the kit. After a simultaneous incubation with a polyclonal antibody to anti-mouse IgG2a conjugated to Horseradish peroxidase, which binds to the mouse IgG2a captured on the plate, the excess reagents are washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of mouse IgG2a in either standards or samples.
- Notes
IgG2 is divided into two subclasses; IgG2a and IgG2b. It is a glycoprotein which consists of two identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig) domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the “hinge region” which is unique to each IgG. This region allows for molecular flexibility and sets IgG2a apart from its IgG counterparts. Properties of IgG2a include neutralization of toxins and diffusion in the extracellular space.
Cross Reactivity
IgG % Cross Reactivity mouse IgG2a 100 mouse IgG3 0.49 mouse IgM 0.12 mouse IgG1 <0.01 mouse IgG2b <0.01 rat IgG2a <0.01 - Tested applicationsELISA more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 1 x 96 tests Assay Buffer 13 Concentrate 1 x 50ml Mouse IgG2a Conjugate 1 x 5ml Mouse IgG2a standard 2 vials Stop Solution 2 1 x 11ml TMB Substrate 1 x 12ml Wash Buffer Concentrate 1 x 100ml - Research Areas
Applications
Our Abpromise guarantee covers the use of ab133046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
IgG2a Mouse ELISA Kit images
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Typical Standard CurveA typical standard curve is shown above. This curve must not be used to calculate mouse IgG2a concentrations; each user must run a standard curve for each assay.
Protocols
References for IgG2a Mouse ELISA Kit (ab133046)
ab133046 has not yet been referenced specifically in any publications.
