11-dehydro-TXB2 ELISA Kit (ab133054)
Overview
- Product name11-dehydro-TXB2 ELISA Kit
- Precision
Intra-assay Sample n Mean SD CV% Low conc 16 67pg/ml 13.2% Medium conc 16 165pg/ml 15.3% High conc 16 318pg/ml 8.1% - Sample typeCell culture supernatant, Urine
- Assay typeCompetitive
- Sensitivity4.31 pg/ml
- Range9.8 pg/ml - 10000 pg/ml
- Recovery
Sample specific recovery Sample type Average % Range Urine 112.2 % - % Tissue Culture Media 104.8 % - % - Assay time3h 0m
- Species reactivityReacts with: Human
Predicted to work with: a wide range of other species - Product overview
ab133054 is a competitive immunoassay for the quantitative determination of 11-dehydro-Thromboxane B2 in tissue culture media and urine. Please read the complete kit insert before performing this assay. The kit for the quantitative measurement of 11-dehydro-Thromboxane B2 uses a polyclonal antibody to 11-dehydro-TXB2 to bind, in a competitive manner, the 11-dehydro-TXB2 in the sample or an alkaline phosphatase molecule which has 11-dehydro-TXB2 covalently attached to it. After a simultaneous incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of 11-dehydro-TXB2 in either standards or samples. The measured optical density is used to calculate the concentration of 11-dehydro-TXB2.
- Notes
11-dehydro-Thromboxane B2 (11-dehydro-TXB2) is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma. It is produced by the dehydrogenation of the alcohol group on the C11 position of TXB2 by the enzyme 11-OH-dehydrogenase. The detection of 11-dehydro-TXB2 is often used to measure thromboxane production in vivo. Monitoring levels of this metabolite is a helpful tool in the study of several diseases, such as liver cirrhosis, cystic fibrosis, mastocytosis, systemic lupus erythematosus, thrombosis diseases, and other diseases involving platelet activation.11-dehydro-TXB2 has also been used in diabetes and asthma studies.
Cross reactivities:
Compound Cross Reactivity 11-dehydro-TXB2 100% PGE2 1.85% TXB2 0.4% PGF2α 0.2% 6-keto-PGF1α 0.17% PGD2 0.1% 2,3-dinor TXB2 0.1% 8-iso-PGF2α 0.07% 11-b-PGF2α 0.05% - Tested applicationsCompetitive ELISA more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 1 x 96 tests 11-dehydro-Thromboxane B2 Standards 1 x 0.5ml 11-dehydro-TXB2 Antibody 1 x 6ml 11-dehydro-TXB2 Conjugate 1 x 6ml Assay Buffer 1 x 30ml Goat anti-Rabbit IgG Microtiter Plate 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml Wash Buffer Concentrate 1 x 30ml - Research Areas
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Alternative names
- 11-dehydro-Thromboxane B2
Applications
Our Abpromise guarantee covers the use of ab133054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Competitive ELISA | cELISA |
11-dehydro-TXB2 ELISA Kit images
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Typical Standard CurveTypical results: This curve must not be used to calculate 11-dehydro-TXB2 concentrations; each user must run a standard curve for each assay.
Protocols
References for 11-dehydro-TXB2 ELISA Kit (ab133054)
ab133054 has not yet been referenced specifically in any publications.
