anti-HSP70 IgG/A/M Human ELISA Kit (ab133063)
- Product nameanti-HSP70 IgG/A/M Human ELISA Kit
- Sample typeSerum
- Assay typeIndirect
- Sensitivity6.79 ng/ml
- Range31.25 ng/ml - 1000 ng/ml
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
ab133063 provides a method to detect and quantitate antibodies to Human Hsp70 in Human serum samples. This assay allows for reproducible, accurate and precise determination of IgG, IgA and IgM antibodies (total) to Human Hsp70.
ab133063 uses recombinant Human Hsp70 pre-coated to the wells of the Rec. Human Hsp70 Immunoassay Plate to capture anti-Human Hsp70 antibodies present in Human serum. The captured anti-Human Hsp70 antibodies are detected with a hydrogen peroxidase conjugated goat polyclonal antibody specific for Human IgG, IgA and IgM molecules. The assay is developed with tetramethylbenzidine substrate producing a blue color in proportion to the amount of captured anti-human Hsp70 antibodies. The color development is stopped with acid stop solution which converts the endpoint color to yellow. The intensity of the color is measured in a microplate reader at 450nm.
ab133063 uses a calibrated standard of anti-human Hsp70 (IgG/A/M) antibodies isolated from pooled human sera to generate a standard curve. The kit provides researchers with a rapid, reliable and standardized method to measure the levels of antihuman Hsp70 antibody levels in human serum samples by interpolating absorbance readings from the standard curve. This kit has the potential of expanding our knowledge of the role of anti-human Hsp70 antibodies in the normal population and as a diagnostic tool to evaluate and monitor a variety of diseases.
The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
- Tested applicationsIndirect ELISA more details
- Storage instructionsPlease refer to protocols.
Components 1 x 96 tests Anti-Human GAM-HRP Conjugate 1 x 10ml Anti-Human Hsp70 Standard 1 x 0.12ml Plate Sealer 1 unit Rec. Human Hsp70 Immunoassay Plate 1 unit Sample Diluent 2 1 x 100ml Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml Wash Buffer Concentrate 1 x 100ml
- Research Areas
- Immunoglobulin AImmunoglobulin GImmunoglobulin M
Our Abpromise guarantee covers the use of ab133063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
anti-HSP70 IgG/A/M Human ELISA Kit images
A typical standard curve is shown below. This curve must not be used to calculate HSP70 IGG/A/M concentrations; each user must run a standard curve for each assay.
References for anti-HSP70 IgG/A/M Human ELISA Kit (ab133063)
ab133063 has not yet been referenced specifically in any publications.