(MS1094)
HeLa Whole Cell Lysate - Calyculin Treated and Untreated Control Set (ab135395)
Overview
- Product nameHeLa Whole Cell Lysate - Calyculin Treated and Untreated Control Set
- Product overview
The treated HeLa whole cell extract was collected in lysis buffer after 3 hour treatment with Calyculin A [50nM] for 3 hours. The protein content was determined by BCA assay. Calyculin A is a potent phosphatase inhibitor. Unlike Okadaic Acid, which only acts on protein phosphatase PP2A, Calyculin A inhibits both PP1 and PP2A. Cells treated with Calyculin A accumulate serine/threonine phosphorylation making them a strong positive control for phospho-threonine or phospho-serine specific antibodies.
- Tested applicationsWB, SDS-PAGE more details
Properties
- Storage instructionsStore at -80°C. Please refer to protocols.
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Components 2 units HeLa Whole Cell Lysate - Calyculin Treated 1 x 200µg HeLa Whole Cell Lysate - Untreated Control 1 x 200µg - Research Areas
Applications
Our Abpromise guarantee covers the use of ab135395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB |
| SDS-PAGE | SDS-PAGE |
HeLa Whole Cell Lysate - Calyculin Treated and Untreated Control Set images
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Western BlotAll lanes: Anti-Phosphoserine/Threonine antibody (ab17464) at 1/1000
Lane 1: Marker
Lane 2: HeLa Whole Cell Lysate - Calyculin Treated at 20 µg
Lane 3: HeLa Whole Cell Lysate – Untreated Control at 20 µg
Secondary:
Goat polyclonal to Mouse IgG – Pre-Adsorbed (HRP) at 1/6000 developed using the ECL technique.
Performed under reducing conditions. -
SDS-PAGELane 1: Marker
Lane 2: HeLa Whole Cell Lysate - Calyculin Treated at 20 µg
Lane 3: HeLa Whole Cell Lysate – Untreated control at 20 µg
Gel
10-20% acrylamide separation gel
References for HeLa Whole Cell Lysate - Calyculin Treated and Untreated Control Set (ab135395)
ab135395 has not yet been referenced specifically in any publications.
