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Overview

  • Product name100x Citrate Buffer pH 6.0See all Citrate buffer reagents ...
  • Tested applicationsIHC-P more details

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferpH: 6.00
    Constituent: 1% Hydrochloric acid
    Note: Buffer 100X concentrated.
  • Concentration information loading...
  • Research Areas
  • RelevanceCitrate buffer is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. Target retrieval prior to IHC procedures obtains positive results, or results in an increase in staining intensity with many primary antibodies.

    • Applications

    Our Abpromise guarantee covers the use of ab64236 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P IHC-P: Use at an assay dependent dilution.

    References for 100x Citrate Buffer pH 6.0 (ab64236)

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab64236 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab64236 Submit a Question for ab64236

    Displaying 1 - 2 of 2 results for Abreviews and Q&A

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    The buffer ab64236 is fine when diluted
    ab64239 the pH changes to 6 when diluted

    Thank you very much for contacting us.

    I have received a reply from a colleague who run the test in lab, for checking the change in pH when this product is diluted. They have confirmed that, with the dilution there was only a fraction of change ...

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    Please send an antigen retrieval protocol for this buffer.

    Thank you for contacting us.

    The basic protocol for using a microwave with this buffer is as follows:

    1. Deparaffinize and rehydrate tissue sections.
    2. Dilute the product 1:100 with deionized water. Example: 2 ml of concentrate...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"