Overview

  • Product name
    100x Tris buffer pH 10.0
    See all Tris buffer reagents
  • Tested applications
    Suitable for: IHC-Pmore details
  • General notes
    Recommended Procedure: Microwave
    1. Deparaffinize and rehydrate tissue sections.
    2. Dilute the product 1:100 with deionized water.
    3. Immerse sections in coplin jar filled with target retrieval solution microwave on HIGH power until boiling.
    4. Keep the slides warm by heating for 10min at LOW power.
    5. Let coplin jar sit in microwave for at least 20min.
    6. Remove and rinse with buffer.
    7. Proceed with staining.

Properties

  • Form
    Liquid
  • Storage instructions
    Store at +4°C.
  • Storage buffer
    pH: 10.00
    Constituent: 30% Tris
    Note: Buffer 100x concentrated.
  • Concentration information loading...
  • Research areas
  • Relevance
    Tris buffer is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. Target retrieval prior to IHC procedures obtains positive results, or results in an increase in staining intensity with many primary antibodies.
  • Alternative names
    • Trishydroxymethylaminomethane

Applications

Our Abpromise guarantee covers the use of ab64251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

References

ab64251 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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