Overview

  • Product name100x Tris buffer pH 10.0
    See all Tris buffer reagents
  • Tested applicationsIHC-Pmore details
  • General notesRecommended Procedure: Microwave
    1. Deparaffinize and rehydrate tissue sections.
    2. Dilute the product 1:100 with deionized water.
    3. Immerse sections in coplin jar filled with target retrieval solution microwave on HIGH power until boiling.
    4. Keep the slides warm by heating for 10min at LOW power.
    5. Let coplin jar sit in microwave for at least 20min.
    6. Remove and rinse with buffer.
    7. Proceed with staining.

Properties

  • RelevanceTris buffer is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. Target retrieval prior to IHC procedures obtains positive results, or results in an increase in staining intensity with many primary antibodies.
  • Alternative names
    • Trishydroxymethylaminomethane

Applications

Our Abpromise guarantee covers the use of ab64251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

References for 100x Tris buffer pH 10.0 (ab64251)

ab64251 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"