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The 10kD Spin Columns (ab93349) are disposable ultrafiltration devices for the concentration of biological samples. They can be used in either a swing bucket or fixed angle rotors which accept 2.0 mL centrifuge tube at maximum speed 10000 x g. The vertical membrane design and thin channel filtration chamber minimizes membrane fouling and provides high speed concentration, even laden solutions. It is specifically designed with low internal surface and membrane area in order to achieve superior recovery from dilute solution.
Maximum initial sample volume is 500µL. The dead stop volume is approximately 20-25µL
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|Concentrator cup: Polycarbonate||1 unit|
|Filtrate vessel: Polypropylene||1 unit|
|Membrane: 10,000 MWCO Polyethersulfone||25 x 500µl|
Our Abpromise guarantee covers the use of ab93349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Purification||Use at an assay dependent concentration.|