• Product name10kD Spin Column
  • Tests
    25 x 1 test
  • Product overview

    The 10kD Spin Columns (ab93349) are disposable ultrafiltration devices for the concentration of biological samples. They can be used in either a swing bucket or fixed angle rotors which accept 2.0 mL centrifuge tube at maximum speed 10000 x g. The vertical membrane design and thin channel filtration chamber minimizes membrane fouling and provides high speed concentration, even laden solutions. It is specifically designed with low internal surface and membrane area in order to achieve superior recovery from dilute solution.

    Maximum initial sample volume is 500µL. The dead stop volume is approximately 20-25µL

    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsSuitable for: Purificationmore details



    Our Abpromise guarantee covers the use of ab93349 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Purification Use at an assay dependent concentration.

    10kD Spin Column images

    References for 10kD Spin Column (ab93349)

    This product has been referenced in:
    • Bogdanovic E  et al. Endoplasmic reticulum stress in adipose tissue augments lipolysis. J Cell Mol Med 19:82-91 (2015). Read more (PubMed: 25381905) »
    • Palamiuc L  et al. A metabolic switch toward lipid use in glycolytic muscle is an early pathologic event in a mouse model of amyotrophic lateral sclerosis. EMBO Mol Med 7:526-46 (2015). Human . Read more (PubMed: 25820275) »

    See all 5 Publications for this product

    Product Wall

    It is hard to predict how long will be required to spin the volume through the spin filter. It depends on how much protein is in the solution to be filtered as higher protein concentrations require longer spin times. I recommend spinning for ...

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    Thank you for contacting us.

    Always ensure that you do not load more than 450 µl of diluted, non-viscous samples. I understand in the case of plasma you may not wish to dilute further. If you still have a difficulty in recovering a reaso...

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    Ich kann bestätigen, dass die "spin cloumns" verwendet werden um alles, das grösser ist als 10kDa loszuwerden.

    Bitte verwenden Sie den deshalb Durchfluss im ATP assay kit.

    The columns have been tested to ensure that the samples are separated correctly. The fragments have been tested using several of our assay kits, verifying that analytes above/below 10 kDa are present in the correct fragment.

    You are exactly correct, insoluble material, whether from cells or tissue, needs to be removed prior to loading into the 10kDa spin column. Thus, after lysis/homogenization, centrifuge your sample to pellet the insoluble material. Then take the superna...

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    We recommend the use of the 10 kDa filters irrespective of your immediate or later usage of the lysed samples. The filters help in separating out proteins which can chew up the analyte. You most likely will not get efficient results without the fliters...

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    Thanks for your response.
    I strongly recommend sticking to the kit protocol. Adding solutions (such as ethanol and perchloric acid) other than those provided in the kit will compromise the efficiency of lactate detection. The centrifugation step ca...

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    Thank you for contacting Abcam.
    Since the lactate dehydrogenase (LDH) in tissue lysates degrades lactate, be sure to keep your samples at -80ºC. Below is our recommended homogenization protocol:
    1. Homogenize the tissue samples (should contain ...

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    Thank you for contacting us.

    Yes, you can definitely use 10 kD spin filter to filter out all the proteins from samples. We routinely use and recommend these filters in conjugation with many of our assays kits.

    I hope this information ...

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    Merci de nous avoir contactés.

    Nous suggérons de mettre votre surnageant (maximum 500 ul) dans la colonne à une vitesse de 10000 x g pour 5 minutes. Par contre les temps varient donc il se peut que 5 minutes ne suffisent ...

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