Overview

Properties

  • RelevanceCitrate buffer is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. Target retrieval prior to IHC procedures obtains positive results, or results in an increase in staining intensity with many primary antibodies.

Applications

Our Abpromise guarantee covers the use of ab64214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

References for 10x Citrate Buffer pH 6.0 (ab64214)

This product has been referenced in:
  • Jujo T  et al. The role of matrix metalloproteinase in the intimal sarcoma-like cells derived from endarterectomized tissues from a chronic thromboembolic pulmonary hypertension patient. PLoS One 9:e87489 (2014). Read more (PubMed: 24489925) »

See 1 Publication for this product

Product Wall

Thank you for your reply.

Your protocol seems comprehensive, although I would strongly recommend including the antigen retrieval step since this has worked well for other customers. We would recommend citrate buffer pH 6, which we do sell as ...

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Thank you for contacting us.

Here is the list with the requested items. However, please be aware that this is not a technical inquiry as all item could be found by using the search application on our website under the "reagents" ca...

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Thank you for contacting us.

We do not currently offer double staining IHC kits. Your staining conditions are challenging as, of the two common HRP chromogens, only DAB is alcohol insoluable. While of the common AP chromagens, only BCIP/NBT ...

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Thank you for your recent telephone enquiry. I can confirm that the concentration of citrate in the buffer is 30 mM. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us. Good lu...

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Thank you for your enquiry. Unfortunately, the concentration of 10 X citrate buffer is proprietary and can not be released. However, we can suggest that you make a ten times dilution of the buffer for the antigen retrieval. Simple take 1ml of this ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"