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10x Citrate Buffer pH 6.0 (ab64214)

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Overview

  • Product name10x Citrate Buffer pH 6.0See all Citrate buffer reagents ...
  • Tested applicationsIHC-P more details

    • Properties

  • FormLiquid
  • Storage instructionsStore at room temperature.
  • Storage bufferpH: 6.00
    Constituent: Citrate Buffer
    Note: Buffer x10 concentrated.
  • Concentration information loading...
  • Research Areas
  • RelevanceCitrate buffer is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. Target retrieval prior to IHC procedures obtains positive results, or results in an increase in staining intensity with many primary antibodies.

    • Applications

    Our Abpromise guarantee covers the use of ab64214 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P IHC-P: Use at an assay dependent dilution.

    References for 10x Citrate Buffer pH 6.0 (ab64214)

    ab64214 has not yet been referenced specifically in any publications.

    Publishing research using ab64214 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab64214 Submit a Question for ab64214

    Displaying 1 - 5 of 5 results for Abreviews and Q&A

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    The tissue I used was rabbit brain tissue. We are specifically looking at cortex. The tissue was harvested via trans cardinal perfusion with PBS and followed by 4% PFA. We then do a 24 hour post fix in the 4% at 4C. After the post fix we put the tissue in...

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    Thank you for your reply.

    Your protocol seems comprehensive, although I would strongly recommend including the antigen retrieval step since this has worked well for other customers. We would recommend citrate buffer pH 6, which we do sell as ab6...

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    Hope you are doing well !

    Please see below an e-mail, we need to do complete Rabies IHC test... i.e all materials from Antibody diluent to retrival to everything..


    Please advice me ASAP.. Also the customer needs specific protoc...

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    Thank you for contacting us.

    Here is the list with the requested items. However, please be aware that this is not a technical inquiry as all item could be found by using the search application on our website under the "reagents" categ...

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    I need to develop double IHC analysis on formalin-fixed paraffin-embedded (FFPE) tissue sections of human tumors specimens, and after we have to do laser capture microdissection of positive cells.   I already have two primary antibodies directed against...

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    Thank you for contacting us.

    We do not currently offer double staining IHC kits. Your staining conditions are challenging as, of the two common HRP chromogens, only DAB is alcohol insoluable. While of the common AP chromagens, only BCIP/NBT is ...

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    ab64214 What is the concentration of the citrate in the buffer?

    Thank you for your recent telephone enquiry. I can confirm that the concentration of citrate in the buffer is 30 mM. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us. Good luck ...

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    How would I make a 0.1M solution of this? I am horrible with dilutions and I'm having a hard time figuring this one out...Any help would be greatly appreciated!　I'm sorry, I think I actually need 10mM. This is for antigen retrieval step in IHC-P (formal...

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    Thank you for your enquiry. Unfortunately, the concentration of 10 X citrate buffer is proprietary and can not be released. However, we can suggest that you make a ten times dilution of the buffer for the antigen retrieval. Simple take 1ml of this sol...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"