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10x Heat Mediated Antigen Retrieval Solution pH 6.0 (ab973)

Submit an Abreview Q&A (2)Specific References (1)

Overview

  • Product name10x Heat Mediated Antigen Retrieval Solution pH 6.0See all Antigen retrieval solution reagents ...
  • Tested applicationsIHC-P more details
  • General notesThis Antigen retrieval solution is citrate buffer pH6. It incorporates ASSURE™, which is a color-coded pH indicator technology. The end-user is assured, by visual inspection, that the solution is at the correct dilution and pH. The 10x solution is orange. The 1X solution is yellow. When boiling, the solution becomes a pale orange. Should the pH rise above 7.0, the solution will turn a reddish color.

    • Properties

  • FormLiquid
  • Storage instructionsStore at room temperature.
  • Storage bufferpH: 7.30
    Preservative: Proprietary preservative
    Constituents: PBS, Carrier protein
    Note: 10x concentrated.
  • Concentration information loading...
  • Reagent notes This Antigen retrieval solution is a 10x buffer solution with paraffin emulsifier, allowing the user to deparaffinize and pretreat tissues in one-step. Xylenes and alcohols are not needed for deparaffinization, thus saving valuable technician time and reducing costs for reagents and hazardous waste. Antibody titers are doubled when compared to citrate buffer, pH 6.0 and background staining is reduced significantly. This product is specially formulated for superior pH stability at high temperature, and will help prevent the possibility of losing pH sensitive antigens.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab973 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P IHC-P: Use at an assay dependent dilution. Please see the protocol page for more details.

    References for 10x Heat Mediated Antigen Retrieval Solution pH 6.0 (ab973)

    This product has been referenced in:
    • Iacobini C  et al. Development of age-dependent glomerular lesions in galectin-3/AGE-receptor-3 knockout mice. Am J Physiol Renal Physiol 289:F611-21 (2005). Read more (PubMed: 15870382) »

    See 1 Publication for this product

    Publishing research using ab973 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab973 Submit a Question for ab973

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    Dear Sir/Madam, I would like to request information regarding one of your products the Heat Mediated Antigen Retrieval Solution ab973. More specifically expiration date, and storage conditions, as I have a flask of 250ml Lot Nº 872611, and am not sure ...

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    Thank you for your enquiry. I can confirm that ab973 10x Heat Mediated Antigen Retrieval Solution pH 6.0 should be stored at room temperature as stated on the datasheet. According to our stock records, the expiration date for Lot Nº 872611 is Dec 11...

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    Hi- With this Ag retrieval solution do you recommend deparaffining and rehydrating prior to HIER? The tech sheet seems to indicate these steps are unnecessary.

    Thank you for your enquiry. You can use the Reveal to deparaffinize the sections. It is important that you use a hot rinse for 10 minutes straight after you have done the retrieval, to make sure that the wax is not redeposited back onto the slide. P...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"