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Products:Kits/ Lysates/ Other >> Tools and Reagents >> IHC Tools/ Reagents
Overview
Product name
10x Heat Mediated Antigen Retrieval Solution pH 6.0
See all Antigen retrieval solution products (3) ...
Tested applications
IHC-Pmore details
General notes
This Antigen retrieval solution is citrate buffer pH6. It incorporates ASSURE™, which is a color-coded pH indicator technology. The end-user is assured, by visual inspection, that the solution is at the correct dilution and pH. The 10x solution is orange. The 1X solution is yellow. When boiling, the solution becomes a pale orange. Should the pH rise above 7.0, the solution will turn a reddish color.
Properties
Storage instructions
Store at room temperature.
Storage buffer
pH: 7.30
Preservative: Proprietary preservative
Constituents: PBS, Carrier protein
Note: 10x concentrated.
Concentration
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Reagent notes
This Antigen retrieval solution is a 10x buffer solution with paraffin emulsifier, allowing the user to deparaffinize and pretreat tissues in one-step. Xylenes and alcohols are not needed for deparaffinization, thus saving valuable technician time and reducing costs for reagents and hazardous waste. Antibody titers are doubled when compared to citrate buffer, pH 6.0 and background staining is reduced significantly. This product is specially formulated for superior pH stability at high temperature, and will help prevent the possibility of losing pH sensitive antigens.
Research areas
Kits/ Lysates/ Other >> Tools and Reagents >> IHC Tools/ Reagents
Applications
Show applications key
Our Abpromise guarantee covers the use of ab973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. (Please see the protocol page for more details.)
Protocols
References for 10x Heat Mediated Antigen Retrieval Solution pH 6.0 (ab973)
This product has been referenced in:
- Iacobini Cet al. Development of age-dependent glomerular lesions in galectin-3/AGE-receptor-3 knockout mice. Am J Physiol Renal Physiol 289:F611-21 (2005).Read more (PubMed: 15870382) »
See 1 publication for this product
Publishing research using ab973? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"