Customer reviews and FAQs

Overview

  • Product name
    10X Annexin V Binding Buffer
    See all Annexin V reagents
  • Tested applications
    Suitable for: Flow Cyt, FMmore details
  • General notes

    The binding buffer contains optimal concentration of calcium which is required for annexin v binding to phosphotidylserine (PS) on the cell surface. This 10X reagent should be diluted to 1X with distilled water (ddH2O) before using in the assay.

    This buffer can be used in conjunction with our Annexin V reagents.

Properties

  • Function
    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Involvement in disease
    Pregnancy loss, recurrent, 3
  • Sequence similarities
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt
  • Alternative names
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PLACENTAL PROTEIN 4
    • Pp4
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all

Applications

Our Abpromise guarantee covers the use of ab14084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
FM Use at an assay dependent concentration.

References for 10X Annexin V Binding Buffer (ab14084)

This product has been referenced in:
  • Grant R  et al. A filtration-based protocol to isolate human Plasma Membrane-derived Vesicles and exosomes from blood plasma. J Immunol Methods 371:143-51 (2011). Read more (PubMed: 21741384) »

See 1 Publication for this product

Publishing research using ab14084? Please let us know so that we can cite the reference in this datasheet.

Product Wall

Submit an abreview for ab14084 Submit a question for ab14084

6 Abreviews or Q&A

10
Votes: Highest First

Dear All,
a customer of ours bought ab14085 (Annexin V apoptosis detection kit), but he needs to buy Annexin V-Fitc and Annexin V binding buffer separately.
Can you please confirm that the following codes are the same included within the kit ...

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Thank you for your inquiry.

I can confirm that ab14082 (5X Annexin V-FITC Apoptosis Detection Reagent) and ab14084 (10X Annexin V Binding Buffer) are the same reagents as in the kit ab14085(Annexin V-FITC Apoptosis Detection Kit).

P...

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Thanks for information. I do not have time to look with whom I should
contact, but I recently ordered ab14084 Annexin-V binding buffer and it
does not work at all, whereas the equal buffers from BD Sciences and
Beckman Coulter worked fin...

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Thank you for taking the time to contact us. Your enquiry has been forwarded to the Scientific Support tem. I am sorry to hear you have had difficulty obtaining satisfactory results when using this buffer.

I would like to reassure you that t...

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Dear Techserve This is the customers wish: Then I would prefer lot GR63048-1.   Thanking you -

This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial of ab14084 as a free of charge replacement. I hope the second bottle will work as it is expected, and please do let me know how you are...

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Please make sure the replacement bottle is from lot#GR48964-2.     Thanks a lot for dealing with this enquiry. Best Regards,

Thank you for your response. I can certainly place a new order for your customer, however regrettably we do no longer have the requested batch: GR48964-2. It has all been sold out. Currently we have  GR59518-1 and GR63048-1 in our stock. Pl...

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We just received this complaint from the customer below:  I ordered Oct, 12 (order 3-4901676515) 2x 50ml Abcam Annexin V 10x Binding Buffer ab14084. I received 2 bottles coming from 2 different lots. Doing my assays end last week the Annexin staining d...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this product. Although the batches are different, both bottl...

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Customer Question: I was wondering what the AnnexinV Binding Buffer is made of.

Thank you for your enquiry. The buffer is made of Tris-based physiological buffer containing the optimal calcium concentration. Please contact us again if you require further information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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