10x Heat Mediated Antigen Retrieval Solution pH 6.0 (ab973)


  • Product name10x Heat Mediated Antigen Retrieval Solution pH 6.0
    See all Antigen retrieval solution reagents
  • Tested applicationsIHC-Pmore details
  • General notesThis Antigen retrieval solution is citrate buffer pH6. It incorporates ASSURE™, which is a color-coded pH indicator technology. The end-user is assured, by visual inspection, that the solution is at the correct dilution and pH. The 10x solution is orange. The 1X solution is yellow. When boiling, the solution becomes a pale orange. Should the pH rise above 7.0, the solution will turn a reddish color.


  • FormLiquid
  • Storage instructionsStore at room temperature.
  • Storage bufferpH: 7.30
    Preservative: Proprietary preservative
    Constituents: PBS, Carrier protein
    Note: 10x concentrated.
  • Concentration information loading...
  • Reagent notesThis Antigen retrieval solution is a 10x buffer solution with paraffin emulsifier, allowing the user to deparaffinize and pretreat tissues in one-step. Xylenes and alcohols are not needed for deparaffinization, thus saving valuable technician time and reducing costs for reagents and hazardous waste. Antibody titers are doubled when compared to citrate buffer, pH 6.0 and background staining is reduced significantly. This product is specially formulated for superior pH stability at high temperature, and will help prevent the possibility of losing pH sensitive antigens.
  • Research areas


    Our Abpromise guarantee covers the use of ab973 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use at an assay dependent dilution. Please see the protocol page for more details.

    References for 10x Heat Mediated Antigen Retrieval Solution pH 6.0 (ab973)

    This product has been referenced in:
    • Wang SK  et al. ITGB6 loss-of-function mutations cause autosomal recessive amelogenesis imperfecta. Hum Mol Genet 23:2157-63 (2014). Read more (PubMed: 24305999) »
    • Iacobini C  et al. Development of age-dependent glomerular lesions in galectin-3/AGE-receptor-3 knockout mice. Am J Physiol Renal Physiol 289:F611-21 (2005). Read more (PubMed: 15870382) »

    See all 2 Publications for this product

    Product Wall

    Te confirmo que el diluyente para llevar la solución 10X a 1X es agua desionizada (1ml solución de recuperación antigénica + 9ml de agua desionizada).

    Thank you for your enquiry. I can confirm that ab973 10x Heat Mediated Antigen Retrieval Solution pH 6.0 should be stored at room temperature as stated on the datasheet. According to our stock records, the expiration date for Lot Nº 872611 is Dec...

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    Thank you for your enquiry. You can use the Reveal to deparaffinize the sections. It is important that you use a hot rinse for 10 minutes straight after you have done the retrieval, to make sure that the wax is not redeposited back onto the slide. ...

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