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Recommended Procedure: Microwave
Deparaffinize and rehydrate tissue sections.
Dilute the product 1:10 with deionized water.
Immerse sections in coplin jar filled with target retrieval solution, microwave on high power until boiling.
Keep the slides warm by heating for 10min at low power.
Let coplin jar sit in microwave for at least 20min.
Remove and rinse with buffer.
Proceed with staining.
Tris buffer is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. Target retrieval prior to IHC procedures obtains positive results, or results in an increase in staining intensity with many primary antibodies.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use at 1x Dilution.
References for 10x Tris Buffer pH 10.0 (ab64222)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"