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Abcam’s 11,12 EET / DHET in vitro ELISA kit is designed for accurate quantitative measurement in serum, plasma, urine, cell culture, tissue samples following proper isolation and purification.
A 96-well plate has been precoated with 11,12 DHET antibody. Samples and the 11,12 DHET-HRP conjugate are added to the wells, where 11,12 DHET in the sample competes with the added 11,12 DHET-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of 11,12 DHET in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|11,12-DHET Standard||1 x 2µl|
|11,12-DHET-HRP Conjugates||1 x 12µl|
|HRP Buffer||1 x 15ml|
|Pre-coated 96-Well Microplate||1 unit|
|Sample Dilution Buffer||1 x 25ml|
|TMB Substrate||1 x 24ml|
|Wash Buffer Solution||1 x 25ml|
Our Abpromise guarantee covers the use of ab175815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
ab175815 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"