Anti-14-3-3 Tau antibody [3B9] (ab10439)

Overview

• Product nameAnti-14-3-3 Tau antibody [3B9]See all 14-3-3 Tau primary antibodies ...
• Description
  Mouse monoclonal [3B9] to 14-3-3 Tau
• Specificityab10439 recognises theta/tau isoform. The antibody does not react with the 14-3-3 zeta isoform.
• Tested applicationsFlow Cyt, IP, WB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Cow, Human
  
• Immunogen

  Recombinant full length protein.

• Positive controlWhole extracts of mouse 3T3, rat PC12, human Jurkat, or normal fibroblasts or bovine MDBK cells. This antibody gave a positive signal during western blot in the following whole cell lysates: MBA MD 231; HeLa; A431; Jurkat; Y79; NIH3T3.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 3mM Sodium Azide
  Constituents: 50% Glycerol, PBS, pH 7.5
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number3B9
• IsotypeIgG1
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • 14-3-3

  • Signal Transduction
  • Protein Phosphorylation
  • pSer / pThr

  • Signal Transduction
  • Adapters
  • Cytoplasmic

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Synapse marker

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Tangles & Tau

Applications

Target

• FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
• Tissue specificityAbundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
• Sequence similaritiesBelongs to the 14-3-3 family.
• Post-translational
  modificationsSer-232 is probably phosphorylated by CK1.
• Cellular localizationCytoplasm. In neurons, axonally transported to the nerve terminals.

Target information above from: UniProt accession P27348 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 768311 Cow
  • Entrez Gene: 10971 Human
  • Entrez Gene: 22630 Mouse
  • Entrez Gene: 25577 Rat
  • Omim: 609009 Human
  • SwissProt: Q3SZI4 Cow
  • SwissProt: P27348 Human
  • SwissProt: P68254 Mouse

  • SwissProt: P68255 Rat
  • Unigene: 74405 Human
  • Unigene: 289630 Mouse
  • Unigene: 458320 Mouse
  • Unigene: 2502 Rat

  see all

• Alternative names
  14 3 3 protein T cell antibody14 3 3 protein tau antibody14 3 3 protein theta antibody

  14 3 3 tau antibody14 3 3 theta antibody14-3-3 Binding Motif antibody14-3-3 protein T-cell antibody14-3-3 protein tau antibody14-3-3 protein theta antibody14-3-3-TAU antibody14-3-3-THETA antibody1433T_HUMAN antibody1C5 antibodyHS1 antibodyKCIP1 antibodyProtein HS1 antibodyProtein kinase C inhibitor protein 1 antibodyProtein tau antibodyT-CELL antibodytyr3/trp5 monooxygenase activation protein, theta antibodyTyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, theta polypeptide antibodyYWHAQ antibody

  see all

Anti-14-3-3 Tau antibody [3B9] images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 Tau antibody [3B9] (ab10439)
  ICC/IF image of ab10439 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10439, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Western blot - Anti-14-3-3 Tau antibody [3B9] (ab10439)
  All lanes : Anti-14-3-3 Tau antibody [3B9] (ab10439)
  
  Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
  Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 5 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
  Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 28 kDa
  Observed band size : 31 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 120 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 1 minute
• 

  Flow Cytometry-Anti-14-3-3 Tau antibody [3B9](ab10439)
  Overlay histogram showing SH-SY5Y cells stained with ab10439 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10439, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.