Anti-14-3-3 Tau antibody (ab52834)

Overview

• Product nameAnti-14-3-3 Tau antibodySee all 14-3-3 Tau primary antibodies ...
• Description
  Mouse polyclonal to 14-3-3 Tau
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse
  Predicted to work with: Rat, Rabbit, Chicken, Cow, Human, Xenopus laevis, Cynomolgus Monkey, Orangutan
• Immunogen

  Vector coding for a partial recombinant fusion protein, corresponding to amino acids 51-151 of Mouse 14-3-3 Tau. Target sequence used to make the antibody: VVGGRRSAWR VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL LDKYLIANAT NPESKVFYLK MKGDYFRYLA EVACGDDRKQ TIENSQGAYQ

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: None
  Constituents: 50% Glycerol, Whole serum
• PurityWhole antiserum
• Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • 14-3-3

  • Signal Transduction
  • Adapters
  • Cytoplasmic

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Synapse marker

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

Applications

Target

• FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
• Tissue specificityAbundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
• Sequence similaritiesBelongs to the 14-3-3 family.
• Post-translational
  modificationsSer-232 is probably phosphorylated by CK1.
• Cellular localizationCytoplasm. In neurons, axonally transported to the nerve terminals.

Target information above from: UniProt accession P27348 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 768311 Cow
  • Entrez Gene: 10971 Human
  • Entrez Gene: 22630 Mouse
  • Entrez Gene: 25577 Rat
  • Omim: 609009 Human
  • SwissProt: Q5ZMD1 Chicken
  • SwissProt: Q3SZI4 Cow
  • SwissProt: P27348 Human

  • SwissProt: P68254 Mouse
  • SwissProt: P68255 Rat
  • SwissProt: Q52M98 Xenopus laevis
  • Unigene: 74405 Human
  • Unigene: 289630 Mouse
  • Unigene: 458320 Mouse
  • Unigene: 2502 Rat
  • Unigene: 46592 Xenopus laevis
  • Unigene: 74593 Xenopus laevis

  see all

• Alternative names
  14 3 3 protein T cell antibody14 3 3 protein tau antibody14 3 3 protein theta antibody

  14 3 3 tau antibody14 3 3 theta antibody14-3-3 protein T-cell antibody14-3-3 protein tau antibody14-3-3 protein theta antibody1433T_HUMAN antibody1C5 antibodyHS1 antibodyKCIP1 antibodyProtein HS1 antibodyProtein kinase C inhibitor protein 1 antibodyProtein tau antibodytyr3/trp5 monooxygenase activation protein, theta antibodyTyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, theta polypeptide antibodyYwhaq antibody

  see all

Anti-14-3-3 Tau antibody images

• 

  Western blot - 14-3-3 Tau antibody (ab52834)
  All lanes : Anti-14-3-3 Tau antibody (ab52834) at 1/1000 dilution
  
  Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
  Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein)
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Lane 1 : Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution
  Lane 2 : Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution
  
  
  Predicted band size : 28 kDa