Overview

  • Product nameAnti-14-3-3 Tau antibody
    See all 14-3-3 Tau primary antibodies
  • Description
    Rabbit polyclonal to 14-3-3 Tau
  • SpecificityDetects endogenous levels of total 14-3-3 Tau protein.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from internal sequence of human 14-3-3 Tau.

  • Positive control
    • Extracts from HUVEC and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
ELISA 1/20000.

Target

  • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
  • Tissue specificityAbundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
  • Sequence similaritiesBelongs to the 14-3-3 family.
  • Post-translational
    modifications
    Ser-232 is probably phosphorylated by CK1.
  • Cellular localizationCytoplasm. In neurons, axonally transported to the nerve terminals.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 protein T cell antibody
    • 14 3 3 protein tau antibody
    • 14 3 3 protein theta antibody
    • 14 3 3 tau antibody
    • 14 3 3 theta antibody
    • 14-3-3 protein T-cell antibody
    • 14-3-3 protein tau antibody
    • 14-3-3 protein theta antibody
    • 1433T_HUMAN antibody
    • 1C5 antibody
    • HS1 antibody
    • KCIP1 antibody
    • Protein HS1 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Protein tau antibody
    • tyr3/trp5 monooxygenase activation protein, theta antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, theta polypeptide antibody
    • Ywhaq antibody
    see all

Anti-14-3-3 Tau antibody images

  • All lanes : Anti-14-3-3 Tau antibody (ab64991) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells at 5-30ug with no immunizing peptide
    Lane 2 : Extracts from HeLa cells at 5-30ug with no immunizing peptide
    Lane 3 : Extracts from HeLa cells at 5-30ug with immunizing peptide at 5-10ug


    Predicted band size : 28 kDa
    Observed band size : 28 kDa

References for Anti-14-3-3 Tau antibody (ab64991)

ab64991 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (Breast)
Loading amount 20 µg
Specification Breast
Gel Running Conditions Non-reduced Denaturing (12 %)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Nov 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"