Anti-14-3-3 Theta + Tau (phospho S232) antibody (ab63369)
Key features and details
- Rabbit polyclonal to 14-3-3 Theta + Tau (phospho S232)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 Theta + Tau (phospho S232) antibody
See all 14-3-3 Theta + Tau primary antibodies -
Description
Rabbit polyclonal to 14-3-3 Theta + Tau (phospho S232) -
Host species
Rabbit -
Specificity
ab63369 detects 14-3-3 Theta + Tau only when phosphorylated at serine 232.
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Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human 14-3-3 Theta + Tau (phospho S232). (S-D-SP-A-G).
Database link: P27348 -
Positive control
- ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/500 - 1/1000.
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Notes |
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ICC/IF
1/500 - 1/1000. |
Target
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
Tissue specificity
Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsSer-232 is probably phosphorylated by CK1. -
Cellular localization
Cytoplasm. In neurons, axonally transported to the nerve terminals. - Information by UniProt
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Database links
- Entrez Gene: 10971 Human
- Entrez Gene: 100503129 Mouse
- Entrez Gene: 22630 Mouse
- Omim: 609009 Human
- SwissProt: P27348 Human
- SwissProt: P68254 Mouse
- Unigene: 611799 Human
- Unigene: 74405 Human
see all -
Alternative names
- 14-3-3 protein T cell antibody
- 14-3-3 protein T-cell antibody
- 14-3-3 protein tau antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab63369 has been referenced in 1 publication.
- McFerrin MB et al. Dysregulation of 14-3-3 proteins in neurodegenerative diseases with Lewy body or Alzheimer pathology. Ann Clin Transl Neurol 4:466-477 (2017). PubMed: 28695147