Anti-14-3-3 Tau (phospho S232) antibody (ab63369)

Overview

• Product nameAnti-14-3-3 Tau (phospho S232) antibodySee all 14-3-3 Tau primary antibodies ...
• Description
  Rabbit polyclonal to 14-3-3 Tau (phospho S232)
• Specificityab63369 detects 14-3-3 Tau only when phosphorylated at serine 232.
• Tested applicationsELISA, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  Synthetic phosphopeptide derived from human 14-3-3 Tau around the phosphorylation site of serine 232 (S-D-S^P-A-G).

• Positive controlHeLa cells.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • 14-3-3

  • Signal Transduction
  • Adapters
  • Cytoplasmic

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Synapse marker

Applications

Target

• FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
• Tissue specificityAbundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
• Sequence similaritiesBelongs to the 14-3-3 family.
• Post-translational
  modificationsSer-232 is probably phosphorylated by CK1.
• Cellular localizationCytoplasm. In neurons, axonally transported to the nerve terminals.

Target information above from: UniProt accession P27348 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10971 Human
  • Entrez Gene: 22630 Mouse
  • Omim: 609009 Human
  • SwissProt: P27348 Human
  • SwissProt: P68254 Mouse
  • Unigene: 74405 Human
  • Unigene: 289630 Mouse
  • Unigene: 458320 Mouse

• Alternative names
  14 3 3 protein T cell antibody14 3 3 protein tau antibody14 3 3 protein theta antibody

  14 3 3 tau antibody14 3 3 theta antibody14-3-3 protein T-cell antibody14-3-3 protein tau antibody14-3-3 protein theta antibody1433T_HUMAN antibody1C5 antibodyHS1 antibodyKCIP1 antibodyProtein HS1 antibodyProtein kinase C inhibitor protein 1 antibodyProtein tau antibodytyr3/trp5 monooxygenase activation protein, theta antibodyTyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, theta polypeptide antibodyYwhaq antibody

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Anti-14-3-3 Tau (phospho S232) antibody images

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  Immunocytochemistry/ Immunofluorescence - 14-3-3 Tau (phospho S232) antibody (ab63369)
  ab63369 at 1/500 dilution staining 14-3-3 Tau in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.