The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa).
1/50 - 1/200.
(using a fluorescent secondary antibody).
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
Belongs to the 14-3-3 family.
The alpha, brain-specific form differs from the beta form in being phosphorylated. Isoform Short contains a N-acetylmethionine at position 1.
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human rectal carcinoma tissue labeling 14-3-3 alpha + beta with ab216618 at 1/200 dilution, followed by Goat anti-Rabbit IgG, PE conjugted at 1/200 dilution.
References for Anti-14-3-3 alpha + beta antibody - C-terminal (ab216618)
has not yet been referenced specifically in any publications.
Publishing research using ab216618? Please let us know so that we can cite the reference in this datasheet.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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