The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa).
1/50 - 1/200.
(using a fluorescent secondary antibody).
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
Belongs to the 14-3-3 family.
The alpha, brain-specific form differs from the beta form in being phosphorylated. Isoform Short contains a N-acetylmethionine at position 1.
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human rectal carcinoma tissue labeling 14-3-3 alpha + beta with ab216618 at 1/200 dilution, followed by Goat anti-Rabbit IgG, PE conjugted at 1/200 dilution.
has not yet been referenced specifically in any publications.
Publishing research using ab216618? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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