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Anti-14-3-3 alpha + beta antibody [Y62] (ab32560)

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Overview

Product name

Anti-14-3-3 alpha + beta antibody [Y62]
See all 14-3-3 alpha + beta products (6) ...

Description

Rabbit monoclonal [Y62] to 14-3-3 alpha + beta

Specificity

This antibody is specific for 14-3-3 alpha + beta.

Tested applications

WB, IHC-P, ICC, IP, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to residues in the C terminal of Human 14-3-3 alpha + beta.

Positive control

WB: Hela cell lysate. IHC-P: Human breast tissue slides.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

Y62

Isotype

IgG

Research areas

Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Stem Cells >> Signaling Pathways >> Wnt >> 14-3-3
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neural Signal Transduction

  • Western blot - 14-3-3 alpha + beta antibody [Y62] (ab32560)Western blot - 14-3-3 alpha + beta antibody [Y62] (ab32560) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 14-3-3 alpha + beta antibody [Y62] (ab32560)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 14-3-3 alpha + beta antibody [Y62] (ab32560) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/1000. Detects a ba...Read more →

    WB: 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/500.

    IHC-P: 1/500.

  • ICC

     ICC: 1/500.

    ICC: 1/500.

  • IP

     IP: 1/40.

    IP: 1/40.

  • Flow Cyt

     Flow Cyt: 1/40.

    Flow Cyt: 1/40.

Target

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.

Sequence similarities

Belongs to the 14-3-3 family.

Post-translational
modifications

The alpha, brain-specific form differs from the beta form in being phosphorylated.
Isoform Short contains a N-acetylmethionine at position 1.

Cellular localization

Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accessionP31946 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • N-terminally processed antibody
  • 14 3 3 alpha antibody
  • 14 3 3 protein beta alpha antibody
  • 14 3 3 protein beta/alpha antibody
  • 14-3-3 protein beta/alpha antibody
  • 1433B_HUMAN antibody
  • Brain protein 14 3 3 beta isoform antibody
  • GW128 antibody
  • HS 1 antibody
  • HS1 antibody
  • KCIP 1 antibody
  • KCIP-1 antibody
  • KCIP1 antibody
  • Protein 1054 antibody
  • Protein kinase C inhibitor protein 1 antibody
  • Tyrosine 3 monooxygenase tryptophan 5 monooxygenase activation protein alpha polypeptide antibody
  • Tyrosine 3 monooxygenase tryptophan 5 monooxygenase activation protein beta polypeptide antibody
  • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation antibody
  • YWHAA antibody
  • YWHAB antibody
see all

Database links

see all

Anti-14-3-3 alpha + beta antibody [Y62] images:

  Western blot - 14-3-3 alpha + beta antibody [Y62] (ab32560)

Western blot - 14-3-3 alpha + beta antibody [Y62] (ab32560)

Anti-14-3-3 alpha + beta antibody [Y62] (ab32560) at 1/1000 dilution + Hela cell lysate.

Predicted band size : 30 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 14-3-3 alpha + beta antibody [Y62] (ab32560)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 14-3-3 alpha + beta antibody [Y62] (ab32560)

Ab32560, at a 1/500 dilution, staining 14-3-3 alpha + beta in paraffin embedded human breast tissue by Immunohistochemistry.

References for Anti-14-3-3 alpha + beta antibody [Y62] (ab32560)

ab32560 has not yet been referenced specifically in any publications.

Publishing research using ab32560? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"