Anti-14-3-3 alpha + beta antibody (ab155032)

Overview

  • Product name
    Anti-14-3-3 alpha + beta antibody
    See all 14-3-3 alpha + beta primary antibodies
  • Description
    Rabbit polyclonal to 14-3-3 alpha + beta
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 89-183 of Human 14-3-3 alpha + beta (UniProt ID: P31946).

  • Positive control
    • 293T, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysate; HeLa cells; Human Ca922 xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab155032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
  • Sequence similarities
    Belongs to the 14-3-3 family.
  • Post-translational
    modifications
    The alpha, brain-specific form differs from the beta form in being phosphorylated.
    Isoform Short contains a N-acetylmethionine at position 1.
  • Cellular localization
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 alpha antibody
    • 14 3 3 protein beta/alpha antibody
    • 14-3-3 protein beta/alpha antibody
    • 1433B_HUMAN antibody
    • Brain protein 14 3 3 beta isoform antibody
    • GW128 antibody
    • HS 1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein 1054 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • YWHAA antibody
    • YWHAB antibody
    see all

Images

  • Anti-14-3-3 alpha + beta antibody (ab155032) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 28 kDa
  • Immunofluorescence analysis of methanol fixed HeLa cells, labeling 14-3-3 alpha + beta using ab155032 at 1/200 dilution. Lower image costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded Human Ca922 xenograft tissue, labeling 14-3-3 alpha + beta using ab155032 at 1/500 dilution.

References

ab155032 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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