Anti-14-3-3 alpha + beta antibody (ab37550)

Overview

• Product nameAnti-14-3-3 alpha + beta antibodySee all 14-3-3 alpha + beta primary antibodies ...
• Description
  Rabbit polyclonal to 14-3-3 alpha + beta
• SpecificityThis antibody binds both transcript variants of 14-3-3 (alpha and beta).
• Tested applicationsELISA, WB, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  A KLH conjugated synthetic peptide selected within aa 200~300 of human 14-3-3 beta.

• Positive controlJurkat cell lysate

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • 14-3-3

  • Signal Transduction
  • Adapters
  • Cytoplasmic

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Other cell division antibodies

Applications

Target

• FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
• Sequence similaritiesBelongs to the 14-3-3 family.
• Post-translational
  modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated.
  Isoform Short contains a N-acetylmethionine at position 1.
• Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accession P31946 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7529 Human
  • Omim: 601289 Human
  • SwissProt: P31946 Human
  • Unigene: 643544 Human

• Alternative names
  14 3 3 alpha antibody14 3 3 protein beta alpha antibody14 3 3 protein beta/alpha antibody

  14-3-3 protein beta/alpha antibody1433B_HUMAN antibodyBrain protein 14 3 3 beta isoform antibodyGW128 antibodyHS 1 antibodyHS1 antibodyKCIP 1 antibodyKCIP-1 antibodyKCIP1 antibodyN-terminally processed antibodyProtein 1054 antibodyProtein kinase C inhibitor protein 1 antibodyTyrosine 3 monooxygenase tryptophan 5 monooxygenase activation protein alpha polypeptide antibodyTyrosine 3 monooxygenase tryptophan 5 monooxygenase activation protein beta polypeptide antibodyTyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation antibodyYWHAA antibodyYWHAB antibody

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Anti-14-3-3 alpha + beta antibody images

• 

  Western blot - 14-3-3 alpha + beta antibody (ab37550)
  Anti-14-3-3 alpha + beta antibody (ab37550) at 1/100 dilution + Jurkat cell lysate
  
  Predicted band size : 28 kDa
  Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 15 kDa,70 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 14-3-3 alpha + beta antibody (ab37550)
  Formalin-fixed and paraffin-embedded human breast cancer tissue stained with ab37550 at a 1/50 dilution (a peroxidase-conjugated secondary antibody was used, followed by AEC staining).

  This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.