Anti-14-3-3 alpha + beta antibody (ab52995)

Overview

  • Product nameAnti-14-3-3 alpha + beta antibody
    See all 14-3-3 alpha + beta primary antibodies
  • Description
    Rabbit polyclonal to 14-3-3 alpha + beta
  • SpecificityBroadly reactive with the ~29-32 kDa members of the 14-3-3 family of proteins. With the exception of a single, conserved amino acid change, this sequence is identical in the delta and zeta isoforms. Three and four conserved amino acid differences are found in the tau and gamma isoforms
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Xenopus laevis, Caenorhabditis elegans
  • Immunogen

    Synthetic peptide derived from the N-terminus of the human 14-3-3 beta/alpha protein

  • Positive control
    • HeLa cell lysate NIH3T3 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab52995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa.
ELISA Use at an assay dependent concentration. The recommended dilution range 0.1-1ug/ml has only been tested in ELISA against the peptide (immunogen). More concentrated antibody might be needed for standard indirect ELISA.

Target

  • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
  • Sequence similaritiesBelongs to the 14-3-3 family.
  • Post-translational
    modifications
    The alpha, brain-specific form differs from the beta form in being phosphorylated.
    Isoform Short contains a N-acetylmethionine at position 1.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 alpha antibody
    • 14 3 3 protein beta/alpha antibody
    • 14-3-3 protein beta/alpha antibody
    • 1433B_HUMAN antibody
    • Brain protein 14 3 3 beta isoform antibody
    • GW128 antibody
    • HS 1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein 1054 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • YWHAA antibody
    • YWHAB antibody
    see all

References for Anti-14-3-3 alpha + beta antibody (ab52995)

ab52995 has not yet been referenced specifically in any publications.

Product Wall

Application ELISA
Sample Human Tissue sections (cerebrospinal fluid)
Specification cerebrospinal fluid
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type Sandwich (Capture)
Username

Dr. M Schmitz

Verified customer

Submitted Aug 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"