Anti-14-3-3 alpha + beta antibody [Y62] (ab32560)

Overview

  • Product nameAnti-14-3-3 alpha + beta antibody [Y62]
    See all 14-3-3 alpha + beta primary antibodies
  • Description
    Rabbit monoclonal [Y62] to 14-3-3 alpha + beta
  • SpecificityThis antibody is specific for 14-3-3 alpha + beta.
  • Tested applicationsSuitable for: WB, IHC-P, ICC, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C terminal of Human 14-3-3 alpha + beta.

  • Positive control
    • WB: Hela cell lysate. IHC-P: Human breast tissue slides.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/500.
IP 1/40.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
  • Sequence similaritiesBelongs to the 14-3-3 family.
  • Post-translational
    modifications
    The alpha, brain-specific form differs from the beta form in being phosphorylated.
    Isoform Short contains a N-acetylmethionine at position 1.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 alpha antibody
    • 14 3 3 protein beta/alpha antibody
    • 14-3-3 protein beta/alpha antibody
    • 1433B_HUMAN antibody
    • Brain protein 14 3 3 beta isoform antibody
    • GW128 antibody
    • HS 1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein 1054 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • YWHAA antibody
    • YWHAB antibody
    see all

Anti-14-3-3 alpha + beta antibody [Y62] images

  • Anti-14-3-3 alpha + beta antibody [Y62] (ab32560) at 1/1000 dilution + Hela cell lysate.

    Predicted band size : 30 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
  • Ab32560, at a 1/500 dilution, staining 14-3-3 alpha + beta in paraffin embedded human breast tissue by Immunohistochemistry.

References for Anti-14-3-3 alpha + beta antibody [Y62] (ab32560)

ab32560 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab32560.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"