The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 30 kDa.
Use at an assay dependent dilution.
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.
Sequence similaritiesBelongs to the 14-3-3 family.
Post-translational modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Isoform Short contains a N-acetylmethionine at position 1.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Western blot - Anti-14-3-3 antibody [3F7] (ab14110)This image is courtesy of an anonymous abreview.
Anti-14-3-3 antibody [3F7] (ab14110) at 1/500 dilution + whole cell lysate prepared from HeLa cells at 10 µg
Secondary HRP anti-mouse IgG at 1/6000 dilution Developed using the ECL technique
Predicted band size : 30 kDa
Exposure time : 25 seconds
This image is courtesy of an anonymous abreview.
Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 antibody [3F7] (ab14110)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France
In the confocal image above are two rat Glia cells side by side with the nuclei labelled with a blue counterstain. Anti-14-3-3 (ab14110) is in red and shows cytosolic and some nuclear localisation. 63x objective, 4x zoom.
Image courtesy of Randal Moldrich (submitted as an abreview).
References for Anti-14-3-3 antibody [3F7] (ab14110)
This product has been referenced in:
Lalle M et al. Dematin, a component of the erythrocyte membrane skeleton, is internalized by the malaria parasite and associates with Plasmodium 14-3-3. J Biol Chem286:1227-36 (2011).
Read more (PubMed: 21084299) »