Recombinant
RabMAb

Anti-14-3-3 antibody [E58] (ab32377)

Overview

  • Product name
    Anti-14-3-3 antibody [E58]
    See all 14-3-3 primary antibodies
  • Description
    Rabbit monoclonal [E58] to 14-3-3
  • Specificity
    100% homology with human 14-3-3 protein beta/alpha (aa12-26, P31946) and 14-3-3 protein zeta/delta (aa10-24, P63104)
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human 14-3-3 (N terminal).

  • Positive control
    • Hela cell lysate and human skin tissue slides.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 30 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    Images

    • ab32377 at a 1:250 dilution staining 14-3-3 alpha + beta in human skin using Immunohistochemistry, Paraffin Embedded Tissue.
    • Anti-14-3-3 antibody [E58] (ab32377) at 1/1000 dilution + Hela cell lysate.

      Predicted band size : 30 kDa
      Observed band size : 30 kDa

    References

    ab32377 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab32377.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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