Overview

  • Product nameAnti-14-3-3 antibody
    See all 14-3-3 primary antibodies
  • Description
    Rabbit polyclonal to 14-3-3
  • SpecificityThis antibody is broadly reactive with members of the 14-3-3 family of proteins (~29–32kDa). With the exception of a single conserved amino acid change, this sequence is identical in the delta and zeta isoforms. Three and four conserved amino acid differences are found in the tau and gamma isoforms, respectively.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Xenopus laevis, Caenorhabditis elegans
  • Immunogen

    A 20 amino acid synthetic peptide derived from the N-terminus of the human 14-3-3 beta/alpha protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab14112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa.
ELISA Use a concentration of 0.1 - 1 µg/ml.

Target

References for Anti-14-3-3 antibody (ab14112)

This product has been referenced in:
  • Fiorillo A  et al. The crystal structure of Giardia duodenalis 14-3-3 in the apo form: when protein post-translational modifications make the difference. PLoS One 9:e92902 (2014). WB ; Human . Read more (PubMed: 24658679) »
  • Lalle M  et al. Interkingdom complementation reveals structural conservation and functional divergence of 14-3-3 proteins. PLoS One 8:e78090 (2013). WB . Read more (PubMed: 24147113) »

See all 6 Publications for this product

Product Wall

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I have been having a look into suitable positive controls to be used with anti-14-3-3 antibody (ab14112).
It seems the protein 14-3-3 is highly expressed in brain tissue and this should therefore serve as a g...

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Thank you for contacting us.

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on

http://www.abcam.com/14-3-3-antibody-ab14112-proto...

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Thank you for your enquiry. I received the following information from the source of the antibody: The detection of 14-3-3 protein in CSF should be relatively easy because of the lower total protein content than serum, ascite or tissue lysates. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"