Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-14-3-3 antibody
See all 14-3-3 products (10) ...
Description
Rabbit polyclonal to 14-3-3
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human, Xenopus laevis
Immunogen
Synthetic peptide corresponding to amino acid residues from the C terminal region of 14-3-3 protein, conjugated to keyhole limpet hemocyanin (KLH). Human.
Positive control
rat brain hippocampus lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 0.1% BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Prepared from rabbit serum by affinity purification via chromatography on an affinity column prepared with the C-terminal peptide used as antigen.
Primary antibody notes
Immunolabeling of the 14-3-3 protein band is completely blocked by pre-adsorption of the antibody with the C-terminal peptide that was used to generate the antibody.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> 14-3-3
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neural Signal Transduction
-
Western blot - 14-3-3 antibody (ab30579)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 30 kDa.
Target
Relevance
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions.
Cellular localization
Cytoplasmic
Alternative names
- 14 3 3 antibody
- 14 3 3 protein beta antibody
- 14 3 3 protein beta/alpha antibody
see all
Database links
- Entrez Gene: 7529 Human
- Entrez Gene: 7534 Human
- Entrez Gene: 54401 Mouse
- Entrez Gene: 22631 Mouse
- Entrez Gene: 56011 Rat
- Entrez Gene: 25578 Rat
- Entrez Gene: 399276 Xenopus laevis
- Entrez Gene: 380457 Xenopus laevis
see all
Anti-14-3-3 antibody images:
Western blot - 14-3-3 antibody (ab30579)
Anti-14-3-3 antibody (ab30579) at 1/1000 dilution + rat brain hippocampus lysate
Predicted band size : 30 kDa
References for Anti-14-3-3 antibody (ab30579)
ab30579 has not yet been referenced specifically in any publications.
Publishing research using ab30579? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"