Overview

  • Product name
    Anti-14-3-3 antibody [CG15]
    See all 14-3-3 primary antibodies
  • Description
    Mouse monoclonal [CG15] to 14-3-3
  • Specificity
    Recognizes human beta, gamma, sigma and zeta isoforms of 14-3-3. The clone number has been updated from (3F7) to (CG15) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein (Human 14-3-3 gamma).

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab14110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 30 kDa.
IP Use at an assay dependent dilution.

Target

  • Function
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.
  • Sequence similarities
    Belongs to the 14-3-3 family.
  • Post-translational
    modifications
    The alpha, brain-specific form differs from the beta form in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
    Isoform Short contains a N-acetylmethionine at position 1.
  • Cellular localization
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 antibody
    • 14 3 3 protein beta antibody
    • 14 3 3 protein beta/alpha antibody
    • 14 3 3 protein zeta antibody
    • 14 3 3 zeta antibody
    • 14-3-3 protein beta/alpha antibody
    • 14-3-3 protein/cytosolic phospholipase A2 antibody
    • 1433B_HUMAN antibody
    • GW128 antibody
    • HS1 antibody
    • KCIP 1 antibody
    • KCIP-1 antibody
    • MGC111427 antibody
    • MGC126532 antibody
    • MGC138156 antibody
    • N-terminally processed antibody
    • Protein 1054 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide antibody
    • Tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide antibody
    • YWHAB antibody
    • YWHAD antibody
    • YWHAZ antibody
    see all

Anti-14-3-3 antibody [CG15] images

  • Anti-14-3-3 antibody [CG15] (ab14110) at 1/500 dilution + whole cell lysate prepared from HeLa cells at 10 µg

    Secondary
    HRP anti-mouse IgG at 1/6000 dilution
    Developed using the ECL technique

    Predicted band size : 30 kDa


    Exposure time : 25 seconds

    This image is courtesy of an anonymous abreview.

  • In the confocal image above are two rat Glia cells side by side with the nuclei labelled with a blue counterstain. Anti-14-3-3 (ab14110) is in red and shows cytosolic and some nuclear localisation. 63x objective, 4x zoom. Image courtesy of Randal Moldrich (submitted as an abreview).

References for Anti-14-3-3 antibody [CG15] (ab14110)

This product has been referenced in:
  • Lalle M  et al. Dematin, a component of the erythrocyte membrane skeleton, is internalized by the malaria parasite and associates with Plasmodium 14-3-3. J Biol Chem 286:1227-36 (2011). WB . Read more (PubMed: 21084299) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF-7)
Loading amount
20 µg
Specification
MCF-7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C
Username

Abcam user community

Verified customer

Submitted Mar 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (lung carcinoma)
Loading amount
40 µg
Specification
lung carcinoma
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Apr 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
10 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (12.5 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 03 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - other (HeLa)
Total protein in input
300 µg
Specification
HeLa
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

Submitted Aug 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Glia cell culture)
Specification
Glia cell culture
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

Submitted Jun 22 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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