Overview

  • Product nameAnti-14-3-3 antibody [E58]
    See all 14-3-3 primary antibodies
  • Description
    Rabbit monoclonal [E58] to 14-3-3
  • Specificity100% homology with human 14-3-3 protein beta/alpha (aa12-26, P31946) and 14-3-3 protein zeta/delta (aa10-24, P63104)
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human 14-3-3 (N terminal).

  • Positive control
    • Hela cell lysate and human skin tissue slides.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab32377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 30 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC or IP.
  • Target

    Anti-14-3-3 antibody [E58] images

    • ab32377 at a 1:250 dilution staining 14-3-3 alpha + beta in human skin using Immunohistochemistry, Paraffin Embedded Tissue.
    • Anti-14-3-3 antibody [E58] (ab32377) at 1/1000 dilution + Hela cell lysate.

      Predicted band size : 30 kDa
      Observed band size : 30 kDa

    References for Anti-14-3-3 antibody [E58] (ab32377)

    ab32377 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"