Overview

  • Product nameAnti-14-3-3 antibody [EPR6380]
    See all 14-3-3 primary antibodies
  • Description
    Rabbit monoclonal [EPR6380] to 14-3-3
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human 14-3-3 aa 200-300.

  • Positive control
    • HeLa, SH-SY5Y, Raji and HepG2 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab125032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.
    • Sequence similaritiesBelongs to the 14-3-3 family.
    • Post-translational
      modifications
      The alpha, brain-specific form differs from the beta form in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
      Isoform Short contains a N-acetylmethionine at position 1.
    • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • 14 3 3 antibody
      • 14 3 3 protein beta antibody
      • 14 3 3 protein beta/alpha antibody
      • 14 3 3 protein zeta antibody
      • 14 3 3 zeta antibody
      • 14-3-3 protein beta/alpha antibody
      • 14-3-3 protein/cytosolic phospholipase A2 antibody
      • 1433B_HUMAN antibody
      • GW128 antibody
      • HS1 antibody
      • KCIP 1 antibody
      • KCIP-1 antibody
      • MGC111427 antibody
      • MGC126532 antibody
      • MGC138156 antibody
      • N-terminally processed antibody
      • Protein 1054 antibody
      • Protein kinase C inhibitor protein 1 antibody
      • Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide antibody
      • Tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide antibody
      • YWHAB antibody
      • YWHAD antibody
      • YWHAZ antibody
      see all

    Anti-14-3-3 antibody [EPR6380] images

    • All lanes : Anti-14-3-3 antibody [EPR6380] (ab125032) at 1/1000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 28 kDa
      Observed band size : 27 kDa (why is the actual band size different from the predicted?)

    References for Anti-14-3-3 antibody [EPR6380] (ab125032)

    ab125032 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"