The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 28 kDa.
Use at an assay dependent dilution.
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
Sequence similaritiesBelongs to the 14-3-3 family.
Post-translational modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated. Isoform Short contains a N-acetylmethionine at position 1.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide antibody
Anti-14-3-3 beta antibody [60C10] images
Western blot - 14-3-3 beta antibody [60C10] (ab16859)
Predicted band size : 28 kDa
ab16859 at 1/2000 dilution staining 14-3-3 beta by Western blot.
Lane 1: HeLa cell lysates.
Lane 2: Mouse brain.
Lane 3: Rat Brain.
References for Anti-14-3-3 beta antibody [60C10] (ab16859)
This product has been referenced in:
Sousa MM et al. An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells. PLoS One8:e55493 (2013).
Read more (PubMed: 23405159) »
Zhang X et al. Resolvin D1 protects podocytes in adriamycin-induced nephropathy through modulation of 14-3-3ß acetylation. PLoS One8:e67471 (2013).
Read more (PubMed: 23840712) »