Anti-14-3-3 beta antibody [J2E9] (ab118963)

Overview

• Product nameAnti-14-3-3 beta antibody [J2E9]See all 14-3-3 beta primary antibodies ...
• Description
  Mouse monoclonal [J2E9] to 14-3-3 beta
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length protein, corresponding to amino acids 1-246 of Human 14-3-3 beta, purified from E. coli (NP_003395.1).

• Positive controlHuman brain cortex, kidney and thyroid tissues; HeLa cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.1% Sodium azide
  Constituent: 99% PBS
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone numberJ2E9
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • 14-3-3

  • Signal Transduction
  • Protein Phosphorylation
  • pSer / pThr

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Synapse marker

Applications

Target

• FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
• Sequence similaritiesBelongs to the 14-3-3 family.
• Post-translational
  modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated.
  Isoform Short contains a N-acetylmethionine at position 1.
• Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accession P31946 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7529 Human
  • Omim: 601289 Human
  • SwissProt: P31946 Human
  • Unigene: 643544 Human

• Alternative names
  14 3 3 protein beta/alpha antibody14-3-3 protein beta/alpha antibody1433B_HUMAN antibody

  Brain protein 14 3 3 beta isoform antibodyGW128 antibodyHS1 antibodyKCIP-1 antibodyKCIP1 antibodyN-terminally processed antibodyProtein 1054 antibodyProtein kinase C inhibitor protein 1 antibodyTyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide antibodyYWHAA antibodyYWHAB antibody

  see all

Anti-14-3-3 beta antibody [J2E9] images

• 

  Western blot - Anti-14-3-3 beta antibody [J2E9] (ab118963)
  Anti-14-3-3 beta antibody [J2E9] (ab118963) at 1/1000 dilution + HeLa cell lysate at 30 µg
  
  Secondary
  Goat anti-Mouse HRP
  developed using the ECL technique
  
  Predicted band size : 28 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody [J2E9] (ab118963)
  ab118963 at 5µg/ml staining 14-3-3 beta in Formalin-fixed, Paraffin-embedded Human brain (cortex) tissue by immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody [J2E9] (ab118963)
  ab118963 at 5µg/ml staining 14-3-3 beta in Formalin-fixed, Paraffin-embedded Human Kidney tissue by immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody [J2E9] (ab118963)
  ab118963 at 5µg/ml staining 14-3-3 beta in Formalin-fixed, Paraffin-embedded Human Thyroid tissue by immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.