Overview

  • Product nameAnti-14-3-3 beta antibody [J2E9]
    See all 14-3-3 beta primary antibodies
  • Description
    Mouse monoclonal [J2E9] to 14-3-3 beta
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein Human 14-3-3 beta.

  • Positive control
    • HeLa cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab82160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

Target

  • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
  • Sequence similaritiesBelongs to the 14-3-3 family.
  • Post-translational
    modifications
    The alpha, brain-specific form differs from the beta form in being phosphorylated.
    Isoform Short contains a N-acetylmethionine at position 1.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 protein beta/alpha antibody
    • 14-3-3 protein beta/alpha antibody
    • 1433B_HUMAN antibody
    • Brain protein 14 3 3 beta isoform antibody
    • GW128 antibody
    • HS1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein 1054 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide antibody
    • YWHAA antibody
    • YWHAB antibody
    see all

Anti-14-3-3 beta antibody [J2E9] images



  • Predicted band size : 28 kDa

    (Top) The HeLa cell lysates (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 beta antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

    (Bottom) The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 beta antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

    Lane 1: A431 cell lysate

  • ICC/IF analysis of 14-3-3 beta in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BI antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488® conjugate (Green).

References for Anti-14-3-3 beta antibody [J2E9] (ab82160)

ab82160 has not yet been referenced specifically in any publications.

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