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Overview

  • Product nameAnti-14-3-3 beta antibody [J2E9]See all 14-3-3 beta primary antibodies ...
  • Description
    Mouse monoclonal [J2E9] to 14-3-3 beta
  • Tested applicationsELISA, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein Human 14-3-3 beta.

  • Positive controlHeLa cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab82160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: Use at an assay dependent dilution.
WB WB: 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

Target

  • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
  • Sequence similaritiesBelongs to the 14-3-3 family.
  • Post-translational
    modifications    The alpha, brain-specific form differs from the beta form in being phosphorylated.
    Isoform Short contains a N-acetylmethionine at position 1.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

    • Target information above from: UniProt accession P31946 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        14 3 3 protein beta/alpha antibody14-3-3 protein beta/alpha antibody1433B_HUMAN antibody
        Brain protein 14 3 3 beta isoform antibodyGW128 antibodyHS1 antibodyKCIP-1 antibodyKCIP1 antibodyN-terminally processed antibodyProtein 1054 antibodyProtein kinase C inhibitor protein 1 antibodyTyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide antibodyYWHAA antibodyYWHAB antibody
      see all

    Anti-14-3-3 beta antibody [J2E9] images

    • Western blot - 14-3-3 beta antibody [J2E9] (ab82160)
      Western blot - 14-3-3 beta antibody [J2E9] (ab82160)
      Anti-14-3-3 beta antibody [J2E9] (ab82160) at 1/1000 dilution + HeLa cell lysate at 30 µg

      Secondary
      goat anti-mouse HRP
      developed using the ECL technique

      Predicted band size : 28 kDa
      Observed band size : 28 kDa

    References for Anti-14-3-3 beta antibody [J2E9] (ab82160)

    ab82160 has not yet been referenced specifically in any publications.

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