Overview

  • Product nameAnti-14-3-3 beta antibody
    See all 14-3-3 beta primary antibodies
  • Description
    Rabbit polyclonal to 14-3-3 beta
  • Tested applicationsICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).

  • Positive control
    • Tested with NIH/3T3 cells, prostate cancer cells and liver tumor cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab15260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent dilution. Predicted molecular weight: 28 kDa.
IP Use at an assay dependent dilution.
IHC-P 1/100.

Target

  • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
  • Sequence similaritiesBelongs to the 14-3-3 family.
  • Post-translational
    modifications
    The alpha, brain-specific form differs from the beta form in being phosphorylated.
    Isoform Short contains a N-acetylmethionine at position 1.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 protein beta/alpha antibody
    • 14-3-3 protein beta/alpha antibody
    • 1433B_HUMAN antibody
    • Brain protein 14 3 3 beta isoform antibody
    • GW 128 antibody
    • GW128 antibody
    • HS 1 antibody
    • HS1 antibody
    • KCIP 1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein 1054 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 Monooxygenase/Tryptophan 5 Monooxygenase Activation Protein Beta Polypeptide antibody
    • YWHAA antibody
    • YWHAB antibody
    see all

Anti-14-3-3 beta antibody images

  • ab15260 staining 14-3-3 beta in human brain by Immunohistochemistry (FFPE-sections).

  • Anti-14-3-3 beta antibody (ab15260) at 1/25 dilution + HeLa cell lysate

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • ICC/IF image of ab15260 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15260, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-14-3-3 beta antibody (ab15260)

ab15260 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Tissue lysate - whole (CNS tissue)
Specification CNS tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 01 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Mouse Tissue lysate - whole (brain tissue)
Specification brain tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 27 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Serum (Cerebrospinal fluid)
Loading amount 15 µg
Specification Cerebrospinal fluid
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mrs. Joanna Gawinecka

Verified customer

Submitted Feb 01 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"