The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
Sequence similaritiesBelongs to the 14-3-3 family.
Post-translational modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated. Isoform Short contains a N-acetylmethionine at position 1.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide antibody
Anti-14-3-3 beta antibody [J2E9] images
Western blot - Anti-14-3-3 beta antibody [J2E9] (ab82160)
Predicted band size : 28 kDa
(Top) The HeLa cell lysates (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 beta antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
(Bottom) The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 beta antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
ICC/IF analysis of 14-3-3 beta in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BI antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488® conjugate (Green).
References for Anti-14-3-3 beta antibody [J2E9] (ab82160)
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