Overview
- Product nameAnti-14-3-3 beta antibody, predilutedSee all 14-3-3 beta primary antibodies ...
- DescriptionRabbit polyclonal to 14-3-3 beta, prediluted
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat - Immunogen
Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).
- Positive controlTested with NIH/3T3 cells, prostate cancer cells and liver tumor cells.
Properties
- FormPrediluted
- Storage instructionsStore at +4°C. Do not freeze.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 1% BSA, 50mM Tris, pH 7.6 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab15262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use at an assay dependent dilution. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). Use neat |
| IHC-P | IHC-P: Use at an assay dependent dilution. Ready-to-use for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
Target
- FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
- Sequence similaritiesBelongs to the 14-3-3 family.
- Post-translational
modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated.
Isoform Short contains a N-acetylmethionine at position 1. - Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
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Database links
- Entrez Gene: 7529 Human
- Entrez Gene: 54401 Mouse
- Entrez Gene: 56011 Rat
- Omim: 601289 Human
- SwissProt: P31946 Human
- SwissProt: Q9CQV8 Mouse
- SwissProt: P35213 Rat
- Unigene: 643544 Human
- Unigene: 34319 Mouse
- Unigene: 485025 Mouse
- Unigene: 8653 Rat
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Target information above from: UniProt accession
P31946
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 14 3 3 protein beta/alpha antibody14-3-3 protein beta/alpha antibody1433B_HUMAN antibody
- Brain protein 14 3 3 beta isoform antibodyGW 128 antibodyGW128 antibodyHS 1 antibodyHS1 antibodyKCIP 1 antibodyKCIP-1 antibodyKCIP1 antibodyN-terminally processed antibodyProtein 1054 antibodyProtein kinase C inhibitor protein 1 antibodyTyrosine 3 Monooxygenase/Tryptophan 5 Monooxygenase Activation Protein Beta Polypeptide antibodyYWHAA antibodyYWHAB antibody
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Anti-14-3-3 beta antibody, prediluted images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 14-3-3 beta antibody, prediluted (ab15262)ab15262 staining 14-3-3 beta in human brain by Immunohistochemistry (FFPE-sections).
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Western blot - 14-3-3 beta antibody, prediluted (ab15262)Anti-14-3-3 beta antibody (ab15260) at 1/25 dilution + HeLa cell lysate.
Predicted band size : 28 kDa
Observed band size : 28 kDa
References for Anti-14-3-3 beta antibody, prediluted (ab15262)
ab15262 has not yet been referenced specifically in any publications.
