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Product name
Anti-14-3-3 beta + zeta antibody [22-IID8B]
See all 14-3-3 beta + zeta products (5) ...
Description
Mouse monoclonal [22-IID8B] to 14-3-3 beta + zeta
Tested applications
WB, ICC, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Dog, Human, Pig, Monkey
Immunogen
Lysed transformed amnion cells (AMA) (Human).
Positive control
HeLa cell lysate.
General notes
Storage below -20°C is not recommended.
For maximum product recovery centrifuge the product vial before removing cap.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Primary antibody notes
14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exist in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.
Clonality
Monoclonal
Clone number
22-IID8B
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> 14-3-3
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Neuroscience >> Neurology process >> Neural Signal Transduction
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 29 , 30 kDa.
ICC: Use at an assay dependent dilution.
IHC-P
IHC-P: 1/50.IHC-P: 1/50.
Target
Relevance
14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.
Cellular localization
Cytoplasmic. Melanosome (fractions from stage I to stage IV)
Alternative names
- 14 3 3 protein beta antibody
- 14 3 3 protein beta/alpha antibody
- 14 3 3 protein zeta/delta antibody
see all
Database links
- Entrez Gene: 286863 Cow
- Entrez Gene: 287022 Cow
- Entrez Gene: 7529 Human
- Entrez Gene: 7534 Human
- Entrez Gene: 54401 Mouse
- Entrez Gene: 22631 Mouse
- Entrez Gene: 56011 Rat
- Entrez Gene: 25578 Rat
see all
References for Anti-14-3-3 beta + zeta antibody [22-IID8B] (ab12347)
This product has been referenced in:
- Kaushal N et al. The regulation of erythropoiesis by selenium in mice. Antioxid Redox Signal 14:1403-12 (2011). WB; Mouse.Read more (PubMed: 20969477) »
- Choi EK et al. Calsenilin is a substrate for caspase-3 that preferentially interacts with the familial Alzheimer's disease-associated C-terminal fragment of presenilin 2. J Biol Chem 276:19197-204 (2001).Read more (PubMed: 11278424) »
See all 3 publications for this product
Publishing research using ab12347? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"