Overview

  • Product nameAnti-14-3-3 beta + zeta antibody [22-IID8B]
    See all 14-3-3 beta + zeta primary antibodies
  • Description
    Mouse monoclonal [22-IID8B] to 14-3-3 beta + zeta
  • Tested applicationsSuitable for: WB, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Dog, Human, Pig, Monkey
  • Immunogen

    Lysed transformed amnion cells (AMA) (Human).

  • Positive control
    • HeLa cell lysate.
  • General notesStorage below -20°C is not recommended. For maximum product recovery centrifuge the product vial before removing cap.


    14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exist in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notes14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exist in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.
  • ClonalityMonoclonal
  • Clone number22-IID8B
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 , 30 kDa.
ICC Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

Target

  • Relevance14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.
  • Cellular localizationCytoplasmic. Melanosome (fractions from stage I to stage IV)
  • Database links
  • Alternative names
    • 14 3 3 protein beta antibody
    • 14 3 3 protein beta/alpha antibody
    • 14 3 3 protein zeta/delta antibody
    • 14 3 3 zeta antibody
    • HS1 antibody
    • KCIP1 antibody
    • YWHAB antibody
    • YWHAZ antibody
    see all

References for Anti-14-3-3 beta + zeta antibody [22-IID8B] (ab12347)

This product has been referenced in:
  • Kaushal N  et al. The regulation of erythropoiesis by selenium in mice. Antioxid Redox Signal 14:1403-12 (2011). WB ; Mouse . Read more (PubMed: 20969477) »
  • Choi EK  et al. Calsenilin is a substrate for caspase-3 that preferentially interacts with the familial Alzheimer's disease-associated C-terminal fragment of presenilin 2. J Biol Chem 276:19197-204 (2001). Read more (PubMed: 11278424) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"