Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody (ab111856)

Overview

  • Product nameAnti-14-3-3 beta + zeta (phospho S184 + S186) antibody
    See all 14-3-3 beta + zeta primary antibodies
  • Description
    Rabbit polyclonal to 14-3-3 beta + zeta (phospho S184 + S186)
  • Specificityab111856 detects endogenous levels of 14-3-3 beta + zeta only when phosphorylated at serine 184 or serine 186.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from Human 14-3-3 beta + zeta around the phosphorylation site of serine 184 or serine 186 (L-N-SP-P-E).

  • Positive control
    • RAW264.7 cell extracts, treated with UV (15mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab111856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.

Target

  • Relevance14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.
  • Cellular localizationCytoplasmic. Melanosome (fractions from stage I to stage IV)
  • Database links
  • Alternative names
    • 14 3 3 protein beta antibody
    • 14 3 3 protein beta/alpha antibody
    • 14 3 3 protein zeta/delta antibody
    • 14 3 3 zeta antibody
    • HS1 antibody
    • KCIP1 antibody
    • YWHAB antibody
    • YWHAZ antibody
    see all

Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody images

  • All lanes : Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody (ab111856) at 1/500 dilution

    Lane 1 : RAW264.7 cell extracts, treated with UV (15mins)
    Lane 2 : RAW264.7 cell extracts, treated with UV (15mins) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 28 kDa

References for Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody (ab111856)

ab111856 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab111856.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"