Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody (ab111856)
Key features and details
- Rabbit polyclonal to 14-3-3 beta + zeta (phospho S184 + S186)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody -
Description
Rabbit polyclonal to 14-3-3 beta + zeta (phospho S184 + S186) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human 14-3-3 beta + zeta (internal sequence) (phospho S184 + S186).
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Positive control
- RAW264.7 cell extracts, treated with UV (15mins).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab111856 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 28 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 28 kDa. |
Target
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Relevance
14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors. -
Cellular localization
Cytoplasmic. Melanosome (fractions from stage I to stage IV) -
Database links
- Entrez Gene: 7529 Human
- Entrez Gene: 7534 Human
- Entrez Gene: 22631 Mouse
- Entrez Gene: 54401 Mouse
- Entrez Gene: 25578 Rat
- Entrez Gene: 56011 Rat
- Omim: 601288 Human
- Omim: 601289 Human
see all -
Alternative names
- 14 3 3 protein beta antibody
- 14 3 3 protein beta/alpha antibody
- 14 3 3 protein zeta/delta antibody
see all
Images
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All lanes : Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody (ab111856) at 1/500 dilution
Lane 1 : RAW264.7 cell extracts, treated with UV (15mins)
Lane 2 : RAW264.7 cell extracts, treated with UV (15mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 28 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab111856 has not yet been referenced specifically in any publications.