Anti-14-3-3 beta + zeta (phospho S185) antibody (ab79166)

Overview

  • Product name
    Anti-14-3-3 beta + zeta (phospho S185) antibody
    See all 14-3-3 beta + zeta primary antibodies
  • Description
    Sheep polyclonal to 14-3-3 beta + zeta (phospho S185)
  • Specificity
    ab79166 recognizes 14-3-3 phospho Serine185. It is specific for the phosphorylated form of beta and zeta 14-3-3 that are found endogenously phosphorylated only in brain tissue.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Cow, Human
  • Immunogen

    Synthetic phosphopeptide corresponding to amino acids surrounding phospho Serine185.

Properties

Applications

Our Abpromise guarantee covers the use of ab79166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/150 - 1/300 using ECL. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.
    • Cellular localization
      Cytoplasmic. Melanosome (fractions from stage I to stage IV)
    • Database links
    • Alternative names
      • 14 3 3 protein beta antibody
      • 14 3 3 protein beta/alpha antibody
      • 14 3 3 protein zeta/delta antibody
      • 14 3 3 zeta antibody
      • HS1 antibody
      • KCIP1 antibody
      • YWHAB antibody
      • YWHAZ antibody
      see all

    References

    ab79166 has not yet been referenced specifically in any publications.

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