Anti-14-3-3 Binding Domain (phospho S) antibody (ab14127)
Key features and details
- Rabbit polyclonal to 14-3-3 Binding Domain (phospho S)
- Suitable for: WB, IP, IHC-P, ELISA
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 Binding Domain (phospho S) antibody -
Description
Rabbit polyclonal to 14-3-3 Binding Domain (phospho S) -
Host species
Rabbit -
Specificity
Ab14127 binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position. Antibody binding is phospho-specific and largely independent of other surrounding amino acids. The antibody weakly crossreacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresponding nonphosphorylated sequences or with other phospho-Thr/Ser/Tyr-containing motifs. By ELISA ab14127 recognizes a wide range of peptides containing the 14-3-3 binding motif and by 2D gel Western blot analysis it recognizes a large number of presumptive 14-3-3 binding proteins. -
Tested applications
Suitable for: WB, IP, IHC-P, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to 14-3-3 Binding Domain (phospho S).
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General notes
This antibody provides a powerful tool for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, BSA -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
This antibody provides a powerful tool for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 30 kDa.
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IP |
1/50.
On denatured proteins. |
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IHC-P |
1/100.
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ELISA |
1/1000.
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Notes |
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WB
1/1000. Predicted molecular weight: 30 kDa. |
IP
1/50. On denatured proteins. |
IHC-P
1/100. |
ELISA
1/1000. |
Target
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Relevance
The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control. Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions. Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins. Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25c, FKHRL1, PKC and c-Raf.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab14127 has been referenced in 1 publication.
- Santpere G et al. Oxidative damage of 14-3-3 zeta and gamma isoforms in Alzheimer's disease and cerebral amyloid angiopathy. Neuroscience 146:1640-51 (2007). WB . PubMed: 17445990