Anti-14-3-3 Binding Domain (phospho S) antibody (ab14127)

Overview

  • Product nameAnti-14-3-3 Binding Domain (phospho S) antibody
  • Description
    Rabbit polyclonal to 14-3-3 Binding Domain (phospho S)
  • SpecificityAb14127 binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position. Antibody binding is phospho-specific and largely independent of other surrounding amino acids. The antibody weakly crossreacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresponding nonphosphorylated sequences or with other phospho-Thr/Ser/Tyr-containing motifs. By ELISA ab14127 recognizes a wide range of peptides containing the 14-3-3 binding motif and by 2D gel Western blot analysis it recognizes a large number of presumptive 14-3-3 binding proteins.
  • Tested applicationsSuitable for: WB, IP, IHC-P, ELISAmore details
  • Immunogen

    Synthetic phospho (Ser) 14-3-3-binding-motif peptides.

  • General notes


    This antibody provides a powerful tool for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, BSA, 150mM Sodium Chloride, 10mM HEPES. pH 7.5
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesThis antibody provides a powerful tool for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 30 kDa.
IP 1/50.

On denatured proteins.

IHC-P 1/100.
ELISA 1/1000.

Target

  • RelevanceThe 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control. Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions. Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins. Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25c, FKHRL1, PKC and c-Raf.

References for Anti-14-3-3 Binding Domain (phospho S) antibody (ab14127)

This product has been referenced in:
  • Santpere G  et al. Oxidative damage of 14-3-3 zeta and gamma isoforms in Alzheimer's disease and cerebral amyloid angiopathy. Neuroscience 146:1640-51 (2007). WB . Read more (PubMed: 17445990) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. I have enquired at the originator of the product but unfortunately, the immunogen sequence for this product is proprietary. Regarding positive controls, human breast carcinoma was used in immunohistochemistry QC for p...

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Thank you for your enquiry. I am afraid we do not have any specific references for this product. However, I was able to find some publications using Hela cells with this clone antibody. I have attached the list below for your reference. 1) Rubio...

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Thank you for your enquiry. It is very rare that an antibody will come with its matching peptide in the same catalogue number product, we typically offer those with a different catalogue number and separate price. For ab14127 it seems we do not sell t...

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Thank you for your enquiry. Your motif has a proline at +2 position, but has a serine at the -3 position instead of the required Arg/Lys. These antibodies have never been tested against your SASSApSLP motif. On the other hand, there is the stateme...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"