Anti-14-3-3 epsilon antibody [EPR3919] (ab108210)

Overview

• Product nameAnti-14-3-3 epsilon antibody [EPR3919]See all 14-3-3 epsilon primary antibodies ...
• Description
  Rabbit monoclonal [EPR3919] to 14-3-3 epsilon
• Tested applicationsWB, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A synthetic peptide corresponding to residues in Human 14-3-3 epsilon.

• Positive controlFetal brain, SW480 and NIH/3T3 cell lysates; HeLa cells
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR3919
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • 14-3-3

  • Signal Transduction
  • Adapters
  • Cytoplasmic

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Synapse marker

Applications

Target

• FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
• Sequence similaritiesBelongs to the 14-3-3 family.
• Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accession P62258 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7531 Human
  • Entrez Gene: 22627 Mouse
  • Entrez Gene: 29753 Rat
  • Omim: 605066 Human
  • SwissProt: P62258 Human
  • SwissProt: P62259 Mouse
  • SwissProt: P62260 Rat
  • Unigene: 513851 Human

  • Unigene: 234700 Mouse
  • Unigene: 471625 Mouse
  • Unigene: 4225 Rat

  see all

• Alternative names
  14 3 3 E antibody14 3 3 epsilon antibody14 3 3E antibody

  14-3-3 protein epsilon antibody14-3-3E antibody1433E_HUMAN antibodyFLJ45465 antibodyFLJ53559 antibodyKCIP 1 antibodyKCIP1 antibodyMDCR antibodyMDS antibodyMitochondrial import stimulation factor L subunit antibodyProtein kinase C inhibitor protein1 antibodyTyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide antibodyTyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide antibodyYWHAE antibody

  see all

Anti-14-3-3 epsilon antibody [EPR3919] images

• 

  Western blot - 14-3-3 epsilon antibody [EPR3919] (ab108210)
  All lanes : Anti-14-3-3 epsilon antibody [EPR3919] (ab108210)
  
  Lane 1 : Fetal brain lysate
  Lane 2 : SW480 cell lysate
  Lane 3 : NIH/3T3 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 29 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - 14-3-3 epsilon antibody [EPR3919] (ab108210)
  ab108210, at a 1/100 dilution, staining 14-3-3 epsilon in HeLa cells by Immunofluorescence.