Anti-14-3-3 epsilon antibody - Carboxyterminal end (ab40117)

Overview

  • Product nameAnti-14-3-3 epsilon antibody - Carboxyterminal end
    See all 14-3-3 epsilon primary antibodies
  • Description
    Rabbit polyclonal to 14-3-3 epsilon - Carboxyterminal end
  • Tested applicationsSuitable for: IP, IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Human
    Predicted to work with: all Mammals
  • Immunogen

    Synthetic peptide from C terminus

Properties

Applications

Our Abpromise guarantee covers the use of ab40117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/3000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA Use at an assay dependent concentration.
ICC/IF 1/400.

Target

  • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Sequence similaritiesBelongs to the 14-3-3 family.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 E antibody
    • 14 3 3 epsilon antibody
    • 14 3 3E antibody
    • 14-3-3 protein epsilon antibody
    • 14-3-3E antibody
    • 1433E_HUMAN antibody
    • Epididymis luminal protein 2 antibody
    • FLJ45465 antibody
    • FLJ53559 antibody
    • HEL2 antibody
    • KCIP 1 antibody
    • KCIP1 antibody
    • MDCR antibody
    • MDS antibody
    • Mitochondrial import stimulation factor L subunit antibody
    • Protein kinase C inhibitor protein1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide antibody
    • YWHAE antibody
    see all

Anti-14-3-3 epsilon antibody - Carboxyterminal end images

  • ICC/IF image of ab40117 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40117, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-14-3-3 epsilon antibody - Carboxyterminal end (ab40117) at 1/500 dilution

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-14-3-3 epsilon antibody - Carboxyterminal end (ab40117)

This product has been referenced in:
  • Tang S  et al. 14-3-3e mediates the cell fate decision-making pathways in response of hepatocellular carcinoma to Bleomycin-induced DNA damage. PLoS One 8:e55268 (2013). Human . Read more (PubMed: 23472066) »
  • Fétaud V  et al. Proteomic profiling in an animal model of acute pancreatitis. Proteomics 8:3621-31 (2008). WB ; Rat . Read more (PubMed: 18686302) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (4-20%)
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (embryo's)
Specification embryo's
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
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Submitted Jan 05 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - nuclear (Hela nuclear extract made by Dignam protocol)
Total protein in input 500 µg
Specification Hela nuclear extract made by Dignam protocol
Immuno-precipitation step Other - Protein G dynabeads
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Submitted Oct 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116 cells)
Loading amount 50 µg
Specification HCT116 cells
Gel Running Conditions Reduced Denaturing (6-15% gradient gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Sep 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"