Overview

  • Product nameAnti-14-3-3 epsilon antibody [EPR3918]
    See all 14-3-3 epsilon primary antibodies
  • Description
    Rabbit monoclonal [EPR3918] to 14-3-3 epsilon
  • Tested applicationsSuitable for: ICC/IF, WB, Flow Cyt, IHC-Frmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human 14-3-3 epsilon (UniProt ID: P62258).

  • Positive control
    • Human fetal brain, SH-SY5Y, 293T and SW480 cell lysates and HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab92311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
Flow Cyt 1/10 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration. PubMed: 25058152
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
    • Sequence similaritiesBelongs to the 14-3-3 family.
    • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • 14 3 3 E antibody
      • 14 3 3 epsilon antibody
      • 14 3 3E antibody
      • 14-3-3 protein epsilon antibody
      • 14-3-3E antibody
      • 1433E_HUMAN antibody
      • Epididymis luminal protein 2 antibody
      • FLJ45465 antibody
      • FLJ53559 antibody
      • HEL2 antibody
      • KCIP 1 antibody
      • KCIP1 antibody
      • MDCR antibody
      • MDS antibody
      • Mitochondrial import stimulation factor L subunit antibody
      • Protein kinase C inhibitor protein1 antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide antibody
      • Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide antibody
      • YWHAE antibody
      see all

    Anti-14-3-3 epsilon antibody [EPR3918] images

    • Overlay histogram showing SH-SY5Y cells stained with ab92311 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92311, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-14-3-3 epsilon antibody [EPR3918] (ab92311) at 1/1000 dilution

      Lane 1 : Human fetal brain cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 29 kDa
    • ab92311 at 1/100 dilution staining 14-3-3 epsilon in HeLa cells by Immunofluorescence.

    References for Anti-14-3-3 epsilon antibody [EPR3918] (ab92311)

    This product has been referenced in:
    • Zhu Y  et al. Neuroprotective effect of TAT-14-3-3e fusion protein against cerebral ischemia/reperfusion injury in rats. PLoS One 9:e93334 (2014). WB ; Rat . Read more (PubMed: 24671253) »
    • Hoyo NL  et al. Functional EF-hands in neuronal calcium sensor GCAP2 determine its phosphorylation state and subcellular distribution in vivo, and are essential for photoreceptor cell integrity. PLoS Genet 10:e1004480 (2014). WB, IHC-Fr ; Mouse . Read more (PubMed: 25058152) »

    See all 3 Publications for this product

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