Recombinant
RabMAb

Anti-14-3-3 eta antibody [EPR16750] (ab206292)

Overview

  • Product name
    Anti-14-3-3 eta antibody [EPR16750]
    See all 14-3-3 eta primary antibodies
  • Description
    Rabbit monoclonal [EPR16750] to 14-3-3 eta
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human 14-3-3 eta aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q04917

  • Positive control
    • WB: Human fetal brain lysate; Jurkat, HeLa, PC-12 and NIH/3T3 whole cell lysates; mouse and rat brain, spleen, heart and kidney lysates; HeLa cytosolic and nuclear fractions. IP: Human brain whole cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR16750
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab206292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP 1/20.

Target

  • Function
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Tissue specificity
    Expressed mainly in the brain and present in other tissues albeit at lower levels.
  • Sequence similarities
    Belongs to the 14-3-3 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 protein eta antibody
    • 14-3-3 eta antibody
    • 14-3-3 protein eta antibody
    • 1433F_HUMAN antibody
    • Brain protein 14-3-3, eta isoform antibody
    • HGNC:12853 antibody
    • Protein AS1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform antibody
    • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
    • YWHA 1 antibody
    • YWHA1 antibody
    • Ywhah antibody
    see all

Images

  • All lanes : Anti-14-3-3 eta antibody [EPR16750] (ab206292) at 1/5000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2 and 3: 3 minutes.

  • All lanes : Anti-14-3-3 eta antibody [EPR16750] (ab206292) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 7 : Mouse heart lysate
    Lane 8 : Mouse kidney lysate
    Lane 9 : Rat heart lysate
    Lane 10 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1- 6 : 30 seconds; Lane 7 -10 : 3 minutes.

  • All lanes : Anti-14-3-3 eta antibody [EPR16750] (ab206292) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cytosolic fraction
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa


    Exposure time : 3 minutes

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • 14-3-3 was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab206292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Negative control: 14-3-3 was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab137106 (which is specific to 14-3-3 gamma) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

References

ab206292 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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