Overview
- Product nameAnti-14-3-3 eta antibodySee all 14-3-3 eta primary antibodies ...
- DescriptionRabbit polyclonal to 14-3-3 eta
- Specificityab118819 may not react with recombinant proteins that are not N-acetylated.
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Xenopus laevis, all Mammals, Cynomolgus Monkey - Immunogen
Synthetic peptide: GDREQLLQRARC, corresponding to acetylated N-terminal amino acids 1-11 of Sheep 14-3-3 eta.
- Positive controlHuman brain, cerebellum and cortex tissues; HEK293 cell lysates.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% Whole serum - PurityWhole antiserum
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab118819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use at an assay dependent dilution. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). |
| IHC-P | IHC-P: 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
- Tissue specificityExpressed mainly in the brain and present in other tissues albeit at lower levels.
- Sequence similaritiesBelongs to the 14-3-3 family.
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Database links
- Entrez Gene: 416955 Chicken
- Entrez Gene: 282126 Cow
- Entrez Gene: 7533 Human
- Entrez Gene: 22629 Mouse
- Entrez Gene: 100328734 Rabbit
- Entrez Gene: 25576 Rat
- Omim: 113508 Human
- SwissProt: P68509 Cow
- SwissProt: Q04917 Human
- SwissProt: P68510 Mouse
- SwissProt: P68511 Rat
- Unigene: 226755 Human
- Unigene: 332314 Mouse
- Unigene: 409488 Mouse
- Unigene: 3324 Rat
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Target information above from: UniProt accession
Q04917
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 14 3 3 protein eta antibody14-3-3 protein eta antibody1433F_HUMAN antibody
- HGNC:12853 antibodyProtein AS1 antibodyTyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibodyTyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide antibodyYWHA 1 antibodyYWHA1 antibodyYwhah antibody
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Anti-14-3-3 eta antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 eta antibody (ab118819)ab118619, at 1/250 dilution, staining 14-3-3 eta in formalin fixed, paraffin embedded Human brain (cerebellum) by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 eta antibody (ab118819)ab118619, at a 1/250 dilution, staining 14-3-3 eta in formalin fixed, paraffin embedded Human brain (cortex) by Immunohistochemistry .followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-14-3-3 eta antibody (ab118819)
ab118819 has not yet been referenced specifically in any publications.
