Overview

  • Product name
  • Description
    Rabbit polyclonal to 14-3-3 eta
  • Specificity
    ab118819 may not react with recombinant proteins that are not N-acetylated.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Xenopus laevis, Mammal, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    GDREQLLQRARC

    , corresponding to acetylated N-terminal amino acids 1-11 of Sheep 14-3-3 eta.

  • Positive control
    • Human brain, cerebellum and cortex tissues; HEK293 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab118819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Tissue specificity
    Expressed mainly in the brain and present in other tissues albeit at lower levels.
  • Sequence similarities
    Belongs to the 14-3-3 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 protein eta antibody
    • 14-3-3 eta antibody
    • 14-3-3 protein eta antibody
    • 1433F_HUMAN antibody
    • Brain protein 14-3-3, eta isoform antibody
    • HGNC:12853 antibody
    • Protein AS1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform antibody
    • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
    • YWHA 1 antibody
    • YWHA1 antibody
    • Ywhah antibody
    see all

Images

  • ab118619, at 1/250 dilution, staining 14-3-3 eta in formalin fixed, paraffin embedded Human brain (cerebellum) by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab118619, at a 1/250 dilution, staining 14-3-3 eta in formalin fixed, paraffin embedded Human brain (cortex) by Immunohistochemistry .followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab118819 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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